Histomorphometric analyses of human adipose tissues using intact, flash-frozen samples.
Histomorphometric analyses of adipose tissue usually require formalin fixation of fresh samples. Our objective was to determine if intact, flash-frozen whole adipose tissue samples stored at − 80 °C could be used for measurements developed for fresh-fixed adipose tissues. Portions of adipose tissue...
| Published in: | Histochemistry & Cell Biology Vol. 149; no. 3; pp. 209 - 219 |
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| Main Authors: | , , , , , , , |
| Format: | Journal Article |
| Published: |
Springer Nature
Mar2018
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=128053664&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 128053664 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09486143 NQI jtl: Histochemistry & Cell Biology issn: 09486143 maglogo: N pubinfo: dt: Mar2018 vid: 149 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 128053664 10.1007/s00418-018-1635-3 128053664 ppf: 209 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Histomorphometric analyses of human adipose tissues using intact, flash-frozen samples. aug: au: Laforest, Sofia Pelletier, Mélissa Michaud, Andréanne Daris, Marleen Descamps, Justine Soulet, Denis Jensen, Michael D. Tchernof, André affil: Endocrinology and Nephrology, CHU de Quebec-Laval University, 2705 Laurier Blvd. (R-4779), G1V 4G2, Quebec, QC, Canada sug: ab: Histomorphometric analyses of adipose tissue usually require formalin fixation of fresh samples. Our objective was to determine if intact, flash-frozen whole adipose tissue samples stored at − 80 °C could be used for measurements developed for fresh-fixed adipose tissues. Portions of adipose tissue samples were either formalin-fixed immediately upon sampling or flash-frozen and stored at − 80 °C and then formalin-fixed during the thawing process. Mean adipocyte diameter was measured. Immunohistochemistry was performed on additional samples to identify macrophage subtypes (M1, CD14 + and M2, CD206 +) and total (CD68 +) number. All slides were counterstained using haematoxylin and eosin (H&E). Visual inspection of H&E-stained adipose tissue slides performed in a blinded fashion showed little or no sign of cell breakage in 74% of frozen-fixed samples and in 68% of fresh-fixed samples (<italic>p</italic> > 0.5). There was no difference in the distribution frequencies of adipocyte sizes in fresh-fixed vs. frozen-fixed tissues in both depots (<italic>p</italic> > 0.9). Mean adipocyte size from frozen-fixed samples correlated significantly and positively with adipocyte size from fresh-fixed samples (<italic>r</italic> = 0.74, <italic>p</italic> < 0.0001, for both depots). The quality of staining/immunostaining and appearance of tissue architecture were comparable in fresh-fixed vs. frozen-fixed samples. In conclusion, intact flash-frozen adipose tissue samples stored at − 80 °C can be used to perform techniques conventionally applied to fresh-fixed samples. This approach allows for retrospective studies with frozen human adipose tissue samples. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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