Identification of urinary metabolites with potential blood pressure-lowering effects in lentil-fed spontaneously hypertensive rats.
Purpose: Urine samples were obtained from a previously completed study that showed lentil consumption attenuates the increase in blood pressure that occurs over time in spontaneously hypertensive rats (SHRs). The objective of the present study was to compare the metabolite profile of the urine sampl...
| Publicado en: | European Journal of Nutrition Vol. 57; no. 1; pp. 297 - 309 |
|---|---|
| Autores principales: | , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Feb2018
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=128149306&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 128149306 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Feb2018 vid: 57 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 128149306 128149306 128149306 10.1007/s00394-016-1319-5 128149306 ppf: 297 ppct: 12 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Identification of urinary metabolites with potential blood pressure-lowering effects in lentil-fed spontaneously hypertensive rats. aug: au: Hanson, Matthew Zahradka, Peter Taylor, Carla G. Aliani, Michel affil: , Department of Physiology and Pathophysiology, University of Manitoba, Winnipeg, Canada sug: subj: Urinalysis Hypertension Blood Pressure Drug Effects Legumes Pharmacodynamics Animal Studies Rats Organic Chemicals Chromatography, High Pressure Liquid Methods Mass Spectrometry Methods Amino Acids Diet Nitric Oxide Arginine Nitric Oxide Synthases ab: Purpose: Urine samples were obtained from a previously completed study that showed lentil consumption attenuates the increase in blood pressure that occurs over time in spontaneously hypertensive rats (SHRs). The objective of the present study was to compare the metabolite profile of the urine samples from control and lentil-fed SHR in relation to the compounds present in lentils but not in other pulses.Methods: The urine samples were from 17-week-old, male SHR fed semi-purified diet prepared with powder (30 %, w/w) from cooked whole pulses or a pulse-free control diet (<italic>n</italic> = 8/group) for 4 weeks. Pulse powders, control diet and urine samples were extracted using acetonitrile and analyzed by a high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-QTOF-MS).Results: Twenty-seven metabolites were significantly different in urine samples from lentil-fed SHR compared to SHR fed control diet, but only 7 were not present in the urine of SHR fed other pulses. Of these metabolites, only citrulline is linked to blood pressure regulation via production of the vasodilator nitric oxide (NO). Several arginine-related compounds that are NO synthase substrates or inhibitors were detected in lentils but not the control diet or other pulse powders.Conclusions: Consumption of lentils increases the availability of arginine and several related compounds that could potentially elevate production of NO and contribute to the blood pressure-lowering effects of lentil-rich diets. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|