Effect of Granulocyte-Colony Stimulating Factor on Endothelial Cells and Osteoblasts.
Objectives. Some animal studies showed that granulocyte-colony stimulating factor (G-CSF) provides beneficial environment for bone healing. It has been well documented that endothelial cells and osteoblasts play critical roles in multiple phases of bone healing. However, the biological effects of G-...
| Publicado en: | BioMed Research International Vol. 2016; pp. 1 - 9 |
|---|---|
| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
1/1/2016
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=128194471&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 128194471 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 1/1/2016 vid: 2016 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 128194471 128194471 128194471 10.1155/2016/8485721 128194471 ppf: 1 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Effect of Granulocyte-Colony Stimulating Factor on Endothelial Cells and Osteoblasts. aug: au: Xi Ling Liu Xiang Hu Wei Xin Cai Lu, Weijia William Li Wu Zheng affil: Discipline of Oral Diagnosis and Polyclinics, Faculty of Dentistry,The University of Hong Kong, Hong Kong sug: subj: Granulocyte Colony-Stimulating Factor Pharmacodynamics Osteoblasts Drug Effects Epithelial Cells Drug Effects Osteogenesis Cell Proliferation Cell Migration Assays Human Granulocyte Colony-Stimulating Factor Administration and Dosage Umbilical Veins Cell Movement Cell Differentiation Bone Development ab: Objectives. Some animal studies showed that granulocyte-colony stimulating factor (G-CSF) provides beneficial environment for bone healing. It has been well documented that endothelial cells and osteoblasts play critical roles in multiple phases of bone healing. However, the biological effects of G-CSF on these cells remain controversial. This study aimed to investigate the influence of G-CSF at various concentrations on endothelial cells and osteoblasts. Materials and Methods. Human umbilical vein endothelial cells (HUVECs) and human osteoblasts (hOBs) were treated with G-CSF at 1000, 100, 10, and 0 ng/mL, respectively. The capacity of cell proliferation, migration, and tube formation of HUVECs was evaluated at 72, 8, and 6 hours after treatment, respectively. The capacity of proliferation, differentiation, and mineralization of hOBs was evaluated at 24 hours, 72 hours, and 21 days after treatment, respectively. Results. HUVECs treated with 100 and 1000 ng/mL G-CSF showed a significantly higher value comparing with controls in migration assay (P < 0.001, P < 0.01, resp.); the group treated with 1000 ng/mL G-CSF showed a significantly lower value on tube formation. No significant difference was detected in groups of hOBs. Conclusions. G-CSF showed favorable effects only on the migration of HUVECs, and no direct influence was found on hOBs. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|