Dihydromyricetin Attenuates TNF-α-Induced Endothelial Dysfunction through miR-21-Mediated DDAH1/ADMA/NO Signal Pathway.
Accumulating studies demonstrate that dihydromyricetin (DMY), a compound extracted from Chinese traditional herb,<italic> Ampelopsis grossedentata</italic>, attenuates atherosclerotic process by improvement of endothelial dysfunction. However, the underlying mechanism remains poorly understood. Thus...
| Publicado en: | BioMed Research International Vol. 2018; pp. 1 - 13 |
|---|---|
| Autores principales: | , , , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
2/28/2018
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=128231472&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 128231472 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2/28/2018 vid: 2018 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 128231472 128231472 128231472 10.1155/2018/1047810 128231472 ppf: 1 ppct: 12 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Dihydromyricetin Attenuates TNF-α-Induced Endothelial Dysfunction through miR-21-Mediated DDAH1/ADMA/NO Signal Pathway. aug: au: Yang, Dafeng Tan, Shenglan Yang, Zhousheng Jiang, Pei Qin, Caie Yuan, Qiong Dang, Ruili Yao, Xiaoxia Qu, Jian Lu, Qiong Xu, Ping Zhang, Bikui Xiang, Daxiong Chen, Lei affil: Department of Pharmacy, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China sug: subj: Flavonols Pharmacodynamics Drugs, Chinese Herbal Pharmacodynamics Plants, Medicinal Tumor Necrosis Factor Endothelium Pathology MicroRNA Drug Effects Hydrolases Drug Effects Arginine Drug Effects Nitric Oxide Drug Effects Signal Transduction Drug Effects Endothelium Drug Effects Epithelial Cells Cell Movement Nitric Oxide Synthases Drug Effects Phosphorylation Gene Expression Enzyme Inhibitors Human Vascular Diseases Drug Therapy Flavonols Therapeutic Use Drugs, Chinese Herbal Therapeutic Use ab: Accumulating studies demonstrate that dihydromyricetin (DMY), a compound extracted from Chinese traditional herb,<italic> Ampelopsis grossedentata</italic>, attenuates atherosclerotic process by improvement of endothelial dysfunction. However, the underlying mechanism remains poorly understood. Thus, the aim of this study is to investigate the potential mechanism behind the attenuating effects of DMY on tumor necrosis factor alpha- (TNF-<italic>α</italic>-) induced endothelial dysfunction. In response to TNF-<italic>α</italic>, microRNA-21 (miR-21) expression was significantly increased in human umbilical vein endothelial cells (HUVECs), in line with impaired endothelial dysfunction as evidenced by decreased tube formation and migration, endothelial nitric oxide synthase (eNOS) (ser1177) phosphorylation, dimethylarginine dimethylaminohydrolases 1 (DDAH1) expression and metabolic activity, and nitric oxide (NO) concentration as well as increased asymmetric dimethylarginine (ADMA) levels. In contrast, DMY or blockade of miR-21 expression ameliorated endothelial dysfunction in HUVECs treated with TNF-<italic>α</italic> through downregulation of miR-21 expression, whereas these effects were abolished by overexpression of miR-21. In addition, using a nonspecific NOS inhibitor, L-NAME, also abrogated the attenuating effects of DMY on endothelial dysfunction. Taken together, these data demonstrated that miR-21-mediated DDAH1/ADMA/NO signal pathway plays an important role in TNF-<italic>α</italic>-induced endothelial dysfunction, and DMY attenuated endothelial dysfunction induced by TNF-<italic>α</italic> in a miR-21-dependent manner. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|