Curcumin as a Promising Antibacterial Agent: Effects on Metabolism and Biofilm Formation in S. mutans.
<italic>Streptococcus mutans (S. mutans)</italic> has been proved to be the main aetiological factor in dental caries. Curcumin, a natural product, has been shown to exhibit therapeutic antibacterial activity, suggesting that curcumin may be of clinical interest. The objective of this study is to ev...
| Publicado en: | BioMed Research International Vol. 2018; pp. 1 - 12 |
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| Autores principales: | , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
2/28/2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=128231477&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 128231477 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2/28/2018 vid: 2018 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 128231477 128231477 128231477 10.1155/2018/4508709 128231477 ppf: 1 ppct: 11 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Curcumin as a Promising Antibacterial Agent: Effects on Metabolism and Biofilm Formation in S. mutans. aug: au: Li, Bingchun Li, Xinlong Lin, Huancai Zhou, Yan affil: Department of Preventive Dentistry, Guanghua School of Stomatology, Sun Yat-sen University, 56 Ling Yuan Road West, Guangzhou 510055, China sug: subj: Curcumin Pharmacodynamics Antiinfective Agents Biofilms Drug Effects Streptococcus Mutans Drug Effects Biological Assay Methods Microscopy Methods Polysaccharides Drug Effects Microscopy, Electron, Scanning Gene Expression Drug Effects Polymerase Chain Reaction Methods Chlorhexidine Microbial Culture and Sensitivity Tests Methods Carbohydrate Metabolism Drug Effects Streptococcal Infections Drug Therapy Curcumin Therapeutic Use ab: <italic>Streptococcus mutans (S. mutans)</italic> has been proved to be the main aetiological factor in dental caries. Curcumin, a natural product, has been shown to exhibit therapeutic antibacterial activity, suggesting that curcumin may be of clinical interest. The objective of this study is to evaluate the inhibitory effects of curcumin on metabolism and biofilm formation in<italic> S. mutans</italic> using a vitro biofilm model in an artificial oral environment.<italic> S. mutans</italic> biofilms were treated with varying concentrations of curcumin. The biofilm metabolism and biofilm biomass were assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and the crystal violet assay. Confocal laser scanning microscopy was used to analyse the composition and extracellular polysaccharide content of<italic> S. mutans</italic> biofilm after curcumin treatment. The biofilm structure was evaluated using a scanning electron microscope. The gene expression of virulence-related factors was assessed by real-time PCR. The antibiofilm effect of curcumin was compared with that of chlorhexidine. The sessile minimum inhibitory concentration (SMIC50%) of curcumin against<italic> S. mutans</italic> biofilm was 500 <italic>μ</italic>M. Curcumin reduced the biofilm metabolism from 5 min to 24 h. Curcumin inhibited the quantity of live bacteria and total bacteria in both the short term (5 min) and the long term. Moreover, curcumin decreased the production of extracellular polysaccharide in the short term. The expression of genes related to extracellular polysaccharide synthesis, carbohydrate metabolism, adherence, and the two-component transduction system decreased after curcumin treatment. The chlorhexidine-treated group showed similar results. We speculate that curcumin has the capacity to be developed as an alternative agent with the potential to reduce the pathogenic traits of<italic> S. mutans</italic> biofilm. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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