A 3D-Printed PLCL Scaffold Coated with Collagen Type I and Its Biocompatibility.
Scaffolds play an important role in tissue engineering and their structure and biocompatibility have great influence on cell behaviors. In this study, poly(l-lactide-co-<italic>ε</italic>-caprolactone) (PLCL) scaffolds were printed by a 3D printing technology, low-temperature deposition manufacturin...
| Publicado en: | BioMed Research International Vol. 2018; pp. 1 - 11 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
2/28/2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=128231478&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 128231478 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2/28/2018 vid: 2018 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 128231478 128231478 128231478 10.1155/2018/5147156 128231478 ppf: 1 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: A 3D-Printed PLCL Scaffold Coated with Collagen Type I and Its Biocompatibility. aug: au: He, Yong Liu, Wei Guan, Lianxiong Chen, Jielin Duan, Li Jia, Zhaofeng Huang, Jianghong Li, Wencui Liu, Jianquan Xiong, Jianyi Liu, Lijun Wang, Daping affil: Department of Orthopedics, The First Hospital Affiliated to Shenzhen University, Shenzhen, Guangdong 518035, China sug: subj: Collagen Printing, Three-Dimensional Polymers Biocompatible Materials Tissue Scaffolds Microscopy, Electron, Scanning Computer-Aided Design Methods Spectrum Analysis Methods Materials Testing Methods Chemical Phenomena Rabbits Animal Studies Chondrocytes Cytological Techniques Methods Cell Proliferation Tissue Engineering Culture Techniques Methods Staining and Labeling Methods ab: Scaffolds play an important role in tissue engineering and their structure and biocompatibility have great influence on cell behaviors. In this study, poly(l-lactide-co-<italic>ε</italic>-caprolactone) (PLCL) scaffolds were printed by a 3D printing technology, low-temperature deposition manufacturing (LDM), and then PLCL scaffolds were treated by alkali and coated with collagen type I (COLI). The scaffolds were characterized by scanning electron microscopy (SEM), porosity test, mechanical test, and infrared spectroscopy. The prepared PLCL and PLCL-COLI scaffolds had three-dimensional (3D) porous structure and they not only have macropores but also have micropores in the deposited lines. Although the mechanical property of PLCL-COLI was slightly lower than that of PLCL scaffold, the hydrophilicity of PLCL-COLI was significantly enhanced. Rabbit articular chondrocytes were extracted and were identified as chondrocytes by toluidine blue staining. To study the biocompatibility, the chondrocytes were seeded on scaffolds for 1, 3, 5, 7, and 10 days. MTT assay showed that the proliferation of chondrocytes on PLCL-COLI scaffold was better than that on PLCL scaffold. And the morphology of cells on PLCL-COLI after 1-day culture was much better than that on PLCL. This 3D-printed PLCL scaffold coated with COLI shows a great potential application in tissue engineering. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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