Evaluation of heme oxygenase 1 (HO 1) in Cd and Ni induced cytotoxicity and crosstalk with ROS quenching enzymes in two to four leaf stage seedlings of <italic>Vigna radiata</italic>.
Research on heme oxygenase in plants has received consideration in recent years due to its several roles in development, defense, and metabolism during various environmental stresses. In the current investigation, the role of heme oxygenase (HO) 1 was evaluated in reducing heavy metal (Cd and Ni) up...
| Publicado en: | Protoplasma Vol. 255; no. 2; pp. 527 - 546 |
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| Autores principales: | , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Mar2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=128255149&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 128255149 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0033183X 2CF jtl: Protoplasma issn: 0033183X maglogo: N pubinfo: dt: Mar2018 vid: 255 iid: 2 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 128255149 10.1007/s00709-017-1166-0 128255149 ppf: 527 ppct: 19 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Evaluation of heme oxygenase 1 (HO 1) in Cd and Ni induced cytotoxicity and crosstalk with ROS quenching enzymes in two to four leaf stage seedlings of <italic>Vigna radiata</italic>. aug: au: Mahawar, Lovely Kumar, Rajesh Shekhawat, Gyan Singh affil: Department of Botany, Jai Narain Vyas University, 342001, Jodhpur, Rajasthan, India sug: ab: Research on heme oxygenase in plants has received consideration in recent years due to its several roles in development, defense, and metabolism during various environmental stresses. In the current investigation, the role of heme oxygenase (HO) 1 was evaluated in reducing heavy metal (Cd and Ni) uptake and alleviating Cd and Ni toxicity effects in the hydroponically grown seedlings of <italic>Vigna radiata</italic> var. <italic>PDM 54.</italic> Seedlings were subjected to Cd- and Ni-induced oxidative stress independently at different concentrations ranging from 10 to 100 μM. After 96 h (fourth day) of treatment, the stressed plants were harvested to study the cellular homeostasis and detoxification mechanism by examining the growth, stress parameters (LPX, H2O2 content), and non-enzymatic and enzymatic parameters (ascorbate peroxidase (APX), guaicol peroxidase (GPX), and catalase (CAT)) including HO 1. At 50 μM CdCl2 and 60 μM NiSO4, HO 1 activity was found to be highest in leaves which were 1.39 and 1.16-fold, respectively. The greatest HO 1 activity was reflected from the reduction of H2O2 content at these metal concentrations (50 μM CdCl2 and 60 μM NiSO4) which is correlated with the increasing activity of other antioxidant enzymes (CAT, APX). Thus, HO 1 works within a group that generates the defense machinery for the plant’s survival by scavenging ROS which is confirmed by a time-dependent study. Hence, it is concluded that seedlings of <italic>V. radiata</italic> were more tolerant towards metal-induced oxidative stress in which HO 1 is localized in its residential area (plastids). pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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