Baicalein Accelerates Tendon-Bone Healing via Activation of Wnt/β-Catenin Signaling Pathway in Rats.
<italic>Background</italic>. Tendon-bone healing is a reconstructive procedure which requires a tendon graft healing to a bone tunnel or to the surface of bone after the junction injury between tendon, ligament, and bone. The surgical reattachment of tendon to bone often fails due to regeneration fa...
| Publicado en: | BioMed Research International Vol. 2018; pp. 1 - 10 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
3/6/2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=128311954&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 128311954 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 3/6/2018 vid: 2018 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 128311954 128311954 128311954 10.1155/2018/3849760 128311954 ppf: 1 ppct: 9 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Baicalein Accelerates Tendon-Bone Healing via Activation of Wnt/β-Catenin Signaling Pathway in Rats. aug: au: Tian, Xinggui Jiang, Huaji Chen, Yuhui Ao, Xiang Chen, Chuan Zhang, Wentao He, Feilin Liao, Xiaoqing Jiang, Xiaocheng Li, Tao Zhang, Zhongmin Zhang, Xintao affil: Department of Orthopedics, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510515, China sug: subj: Signal Transduction Tendons Surgery Bone and Bones Surgery Wnt Proteins Analysis Cytoskeletal Proteins Analysis Flavones Administration and Dosage Animal Studies Rats Grafts Models, Biological Cell Differentiation In Vivo Studies Fibroblasts In Vitro Studies Alkaline Phosphatase Transcription Factors Osteocalcin Collagen ab: <italic>Background</italic>. Tendon-bone healing is a reconstructive procedure which requires a tendon graft healing to a bone tunnel or to the surface of bone after the junction injury between tendon, ligament, and bone. The surgical reattachment of tendon to bone often fails due to regeneration failure of the specialized tendon-bone junction.<italic> Materials and Methods</italic>. An extra-articular tendon-bone healing rat model was established to discuss the effect of the baicalein 10 mg/(kg·d) in accelerating tendon-bone healing progress. Also, tendon-derived stem cells (TDSCs) were treated with various concentrations of baicalein or dickkopf-1 (DKK-1) to stimulate differentiation for 14 days.<italic> Results</italic>.<italic> In vivo,</italic> tendon-bone healing strength of experiment group was obviously stronger than the control group in 3 weeks as well as in 6 weeks. And there were more mature fibroblasts, more Sharpey fibers, and larger new bone formation area treated intragastrically with baicalein compared with rats that were treated with vehicle for 3 weeks and 6 weeks.<italic> In vitro,</italic> after induction for 14 days, the expressions of osteoblast differentiation markers, that is, alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), osteocalcin (OCN), osterix (OSX), and collagen I, were upregulated and Wnt/<italic>β</italic>-catenin signaling pathway was enhanced in TDSCs. The effect of DKK-1 significantly reduced the effect of baicalein on the osteogenic differentiation.<italic> Conclusion</italic>. These data suggest that baicalein may stimulate TDSCs osteogenic differentiation via activation of Wnt/<italic>β</italic>-catenin signaling pathway to accelerate tendon-bone healing. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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