Digital image analysis agrees with visual estimates of adult bone marrow trephine biopsy cellularity.
Abstract: Introduction: Evaluation of cellularity is an essential component of bone marrow trephine biopsy examination. The standard practice is to report the results as visual estimates (VE). Digital image analysis (DIA) offers the promise of more objective measurements of cellularity. Methods: Adu...
| Publicado en: | International Journal of Laboratory Hematology Vol. 40; no. 2; pp. 209 - 215 |
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| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Apr2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=128458872&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 128458872 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17515521 47EA jtl: International Journal of Laboratory Hematology issn: 17515521 maglogo: Y pubinfo: dt: Apr2018 vid: 40 iid: 2 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 128458872 128458872 128458872 10.1111/ijlh.12768 128458872 ppf: 209 ppct: 6 formats: tig: atl: Digital image analysis agrees with visual estimates of adult bone marrow trephine biopsy cellularity. aug: au: Hagiya, A. S. Etman, A. Siddiqi, I. N. Cen, S. Matcuk, Jr, G. R. Brynes, R. K. Salama, M. E. affil: Department of Pathology, Los Angeles County‐USC Medical Center, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA sug: subj: Digital Imaging Visual Perception Bone Marrow In Adulthood Biopsy Methods Human Intraclass Correlation Coefficient Data Analysis Software Magnetic Resonance Imaging Interrater Reliability Prospective Studies Adult Adult: 19-44 years ab: Abstract: Introduction: Evaluation of cellularity is an essential component of bone marrow trephine biopsy examination. The standard practice is to report the results as visual estimates (VE). Digital image analysis (DIA) offers the promise of more objective measurements of cellularity. Methods: Adult bone marrow trephine biopsy sections were assessed for cellularity by VE. Sections were scanned using an Aperio AT2 Scanscope and analyzed using a Cytonuclear (version 1.4) algorithm on halo software. Intraclass correlation (ICC) was used to assess relatedness between VE and DIA, and between MRI and DIA for a separate subset of patients. Trephine biopsy sections from a subset of patients with bone marrow biopsies uninvolved by malignancy were assessed for age‐related changes. Results: Interobserver VE agreement was good to excellent. The ICC value was 0.81 for VE and DIA, and 0.50 for MRI and DIA. Linearity studies showed no statistically significant trend for age‐related changes in cellularity in our cohort (<italic>r</italic> = −.29, <italic>P</italic> = .06). Conclusions: Agreement was good between VE and DIA. It may be possible to use DIA or VE to measure cellularity in the appropriate clinical scenario. The limited sample size precludes similar determinations for MRI calculations. Further studies examining healthy donors are necessary before making definitive conclusions regarding age and cellularity. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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