Optical coherence tomography for assessment of epithelialization in a human ex vivo wound model.
The ex vivo human skin wound model is a widely accepted model to study wound epithelialization. Due to a lack of animal models that fully replicate human conditions, the ex vivo model is a valuable tool to study mechanisms of wound reepithelialization, as well as for preclinical testing of novel the...
| Publicado en: | Wound Repair & Regeneration Vol. 25; no. 6; pp. 1017 - 1027 |
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| Autores principales: | , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Nov/Dec2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=128531895&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 128531895 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Nov/Dec2017 vid: 25 iid: 6 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 128531895 128531895 128531895 10.1111/wrr.12600 128531895 ppf: 1017 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Optical coherence tomography for assessment of epithelialization in a human ex vivo wound model. aug: au: Glinos, George D. Verne, Sebastian H. Aldahan, Adam S. Liang, Liang Nouri, Keyvan Elliot, Sharon Glassberg, Marilyn Cabrera DeBuc, Delia Koru‐Sengul, Tulay Tomic‐Canic, Marjana Pastar, Irena affil: Department of Dermatology and Cutaneous Surgery, Wound Healing and Regenerative Medicine Research Program, Miami, Florida sug: subj: Tomography, Optical Coherence Wound Care Epithelial Cells Models, Biological Human Skin Wound Healing Evaluation Diffusion of Innovation Biopsy Diagnostic Imaging Funding Source ab: The ex vivo human skin wound model is a widely accepted model to study wound epithelialization. Due to a lack of animal models that fully replicate human conditions, the ex vivo model is a valuable tool to study mechanisms of wound reepithelialization, as well as for preclinical testing of novel therapeutics. The current standard for assessment of wound healing in this model is histomorphometric analysis, which is labor intensive, time consuming, and requires multiple biological and technical replicates in addition to assessment of different time points. Optical coherence tomography (OCT) is an emerging noninvasive imaging technology originally developed for noninvasive retinal scans that avoids the deleterious effects of tissue processing. This study investigated OCT as a novel method for assessing reepithelialization in the human ex vivo wound model. Excisional ex vivo wounds were created, maintained at air‐liquid interface, and healing progression was assessed at days 4 and 7 with OCT and histology. OCT provided adequate resolution to identify the epidermis, the papillary and reticular dermis, and importantly, migrating epithelium in the wound bed. We have deployed OCT as a noninvasive tool to produce, longitudinal “optical biopsies” of ex vivo human wound healing process, and we established an optimal quantification method of re‐epithelialization based on en face OCT images of the total wound area. Pairwise statistical analysis of OCT and histology based quantifications for the rate of epithelialization have shown the feasibility and superiority of OCT technology for noninvasive monitoring of human wound epithelialization. Furthermore, we have utilized OCT to evaluate therapeutic potential of allogeneic adipose stem cells revealing their ability to promote reepithelialization in human ex vivo wounds. OCT technology is promising for its applications in wound healing and evaluation of novel therapeutics in both the laboratory and the clinical settings. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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