Evaluation of the aroA mutant of Corynebacterium pseudotuberculosis in cellular and murine models.
Caseous lymphadenitis of small ruminants causes economic losses worldwide. To date, no effective vaccine has been developed against the causative agent of this disease, Corynebacterium pseudotuberculosis. The objective of the present work was to evaluate an aroA mutant gene strain of C. pseudotuberc...
| Publicado en: | Veterinaria México OA Vol. 3; no. 4; pp. 1 - 15 |
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| Autores principales: | , , , , |
| Formato: | Artículo |
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Facultad de Medicina Veterinaria y Zootecnia UNAM
oct-dic2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=128595386&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 128595386 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 24486760 NFHU jtl: Veterinaria México OA issn: 24486760 maglogo: N pubinfo: dt: oct-dic2016 vid: 3 iid: 4 pid: 20869 pub: Facultad de Medicina Veterinaria y Zootecnia UNAM artinfo: ui: 128595386 10.21753/vmoa.3.4.366 ppf: 1 ppct: 14 formats: fmt: @attributes: type: P size: 369KB tig: atl: Evaluation of the aroA mutant of Corynebacterium pseudotuberculosis in cellular and murine models. aug: au: Ibarra Zazueta, Cristina Arellano Reynoso, Beatriz Hernández Castro, Rigoberto Palomares Resendiz, Erika Gabriela Díaz Aparicio, Efrén affil: Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Circuito Exterior S/N, Ciudad Universitaria, Coyoacán 04510, Ciudad de México, México Departamento de Ecología y agentes patógenos, Hospital General Dr. Manuel Gea González, Tlalpan, 14080, Ciudad de México, México CENID Microbiología Animal INIFAP, Cuajimalpa, 05110, Ciudad de México, México su: Corynebacterium pseudotuberculosis Lymphadenitis Pseudotuberculosis Vaccination Mice sug: subj: Corynebacterium pseudotuberculosis Lymphadenitis Pseudotuberculosis Vaccination Mice keyword: aroA gene cellular model murine model vaccination ab: Caseous lymphadenitis of small ruminants causes economic losses worldwide. To date, no effective vaccine has been developed against the causative agent of this disease, Corynebacterium pseudotuberculosis. The objective of the present work was to evaluate an aroA mutant gene strain of C. pseudotuberculosis in cellular and murine models, for attenuation and the ability to stimulate an immune response. The intracellular survival of the aroA mutant strain and the wild type strain (WT) of C. pseudotuberculosis was evaluated in J774A.1 murine macrophages using a multiplicity of infection (MOI) of 1:1 with the following infection times: 30 min, and 1, 2, 4, 8, 12, and 24 h. The largest difference in the intracellular survival of the mutant was observed 30 min post-infection. After subcutaneous skin vaccination, the subcutaneous lesion progression observed on the 14 day was more severe in those animals that were vaccinated with the WT strain. An analysis of the residual virulence in the murine model did not reveal any bacteria in mice vaccinated with the aroA strain on day 28 post-vaccination. Mice vaccinated with the mutant showed 50 % protection against the intraperitoneal challenge, exceeding that of the control group (41.67 %). We conclude that the virulence of the aroA mutant was significantly attenuated in both cellular and murine models according to the residual virulence detected in mice. However, vaccination with the mutant failed to confer at least 80 % protection, which is desirable for an immunogen. Hence, this study contributes to the knowledge of the immune response against Corynebacterium pseudotuberculosis. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y custom: Copyright of Veterinaria México OA is the property of Facultad de Medicina Veterinaria y Zootecnia UNAM and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: Veterinaria México OA holder: Facultad de Medicina Veterinaria y Zootecnia UNAM dt: @attributes: year: 2016 holdings: @attributes: islocal: N |
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