[18F]DPA-714 PET Imaging Reveals Global Neuroinflammation in Zika Virus-Infected Mice.
Purpose: The association of Zika virus (ZIKV) infection and development of neurological sequelae require a better understanding of the pathogenic mechanisms causing severe disease. The purpose of this study was to evaluate the ability and sensitivity of positron emission tomography (PET) imaging usi...
| Publicado en: | Molecular Imaging & Biology Vol. 20; no. 2; pp. 275 - 284 |
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| Autores principales: | , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Apr2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=128617551&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 128617551 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Apr2018 vid: 20 iid: 2 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 128617551 128617551 NLM28900831 128617551 10.1007/s11307-017-1118-2 NLM28900831 128617551 ppf: 275 ppct: 9 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: [18F]DPA-714 PET Imaging Reveals Global Neuroinflammation in Zika Virus-Infected Mice. aug: au: Kuszpit, Kyle Hollidge, Bradley S. Zeng, Xiankun Stafford, Robert G. Daye, Sharon Zhang, Xiang Basuli, Falguni Golden, Joseph W. Swenson, Rolf E. Smith, Darci R. Bocan, Thomas M. affil: Molecular and Translational Sciences Division, U.S. Army Medical Research Institute of Infectious Diseases, 1425, Porter St., Ft. Detrick, 21702, Frederick, MD, USA sug: subj: Inflammation Tomography, Emission-Computed Heterocyclic Compounds Brain Pathology Fluorine Radioisotopes RNA Metabolism Pathologic Processes Pathology Pathologic Processes Blood Inflammation Blood Mice Female Inflammation Pathology Brain Receptors, Cell Surface Metabolism Animal Studies Validation Studies Comparative Studies Evaluation Research Multicenter Studies Funding Source Female ab: Purpose: The association of Zika virus (ZIKV) infection and development of neurological sequelae require a better understanding of the pathogenic mechanisms causing severe disease. The purpose of this study was to evaluate the ability and sensitivity of positron emission tomography (PET) imaging using [18F]DPA-714, a translocator protein (TSPO) 18 kDa radioligand, to detect and quantify neuroinflammation in ZIKV-infected mice.Procedures: We assessed ZIKV-induced pathogenesis in wild-type C57BL/6 mice administered an antibody to inhibit type I interferon (IFN) signaling. [18F]DPA-714 PET imaging was performed on days 3, 6, and 10 post-infection (PI), and tissues were subsequently processed for histological evaluation, quantification of microgliosis, and detection of viral RNA by in situ hybridization (ISH).Results: In susceptible ZIKV-infected mice, viral titers in the brain increased from days 3 to 10 PI. Over this span, these mice showed a two- to sixfold increase in global brain neuroinflammation using [18F]DPA-714 PET imaging despite limited, regional detection of viral RNA. No measurable increase in ionized calcium binding adaptor molecule 1 (Iba-1) expression was noted at day 3 PI; however, there was a modest increase at day 6 PI and an approximately significant fourfold increase in Iba-1 expression at day 10 PI in the susceptible ZIKV-infected group relative to controls.Conclusions: The results of the current study demonstrate that global neuroinflammation plays a significant role in the progression of ZIKV infection and that [18F]DPA-714 PET imaging is a sensitive tool relative to histology for the detection of neuroinflammation. [18F]DPA-714 PET imaging may be useful in dynamically characterizing the pathology associated with neurotropic viruses and the evaluation of therapeutics being developed for treatment of infectious diseases. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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