Pilot Study of an Open-source Image Analysis Software for Automated Screening of Conventional Cervical Smears.

Introduction: The Pap stained cervical smear is a screening tool for cervical cancer. Commercial systems are used for automated screening of liquid based cervical smears. However, there is no image analysis software used for conventional cervical smears. The aim of this study was to develop and test...

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Publicado en:Journal of Cytology Vol. 35; no. 2; pp. 71 - 75
Autores principales: Sanyal, Parikshit, Ganguli, Prosenjit, Barui, Sanghita, Deb, Prabal
Formato: algorithm pictorial research tables/charts Journal Article
Publicado: Wolters Kluwer India Pvt Ltd Apr-Jun2018
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Apr-Jun2018
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      pub: Wolters Kluwer India Pvt Ltd
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        10.4103/JOC.JOC_110_17
        128658257
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        atl: Pilot Study of an Open-source Image Analysis Software for Automated Screening of Conventional Cervical Smears.
      aug:
        au:
          Sanyal, Parikshit
          Ganguli, Prosenjit
          Barui, Sanghita
          Deb, Prabal
        affil: Department of Pathology, Command Hospital (EC), Alipore, Kolkata, West Bengal
      sug:
        subj:
          Image Processing, Computer Assisted
          Software
          Automation
          Cancer Screening
          Cervix Neoplasms Diagnosis
          Pilot Studies
          Cervical Smears Evaluation
          Validity
          Human
          Descriptive Statistics
          Cell Nucleus
          Cytoplasm
          Neutrophils
          Programming Languages
          Software Design
          Validation Studies
          Female
          Female
      ab: Introduction: The Pap stained cervical smear is a screening tool for cervical cancer. Commercial systems are used for automated screening of liquid based cervical smears. However, there is no image analysis software used for conventional cervical smears. The aim of this study was to develop and test the diagnostic accuracy of a software for analysis of conventional smears. Materials and Methods: The software was developed using Python programming language and open source libraries. It was standardized with images from Bethesda Interobserver Reproducibility Project. One hundred and thirty images from smears which were reported Negative for Intraepithelial Lesion or Malignancy (NILM), and 45 images where some abnormality has been reported, were collected from the archives of the hospital. The software was then tested on the images. Results: The software was able to segregate images based on overall nuclear: cytoplasmic ratio, coefficient of variation (CV) in nuclear size, nuclear membrane irregularity, and clustering. 68.88% of abnormal images were flagged by the software, as well as 19.23% of NILM images. The major difficulties faced were segmentation of overlapping cell clusters and separation of neutrophils. Conclusion: The software shows potential as a screening tool for conventional cervical smears; however, further refinement in technique is required.
      pubtype: Academic Journal
      doctype:
        algorithm
        pictorial
        research
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        Journal Article
      ougenre: Article
    language: English
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