Noninvasive reconstruction of placental methylome from maternal plasma DNA: Potential for prenatal testing and monitoring.
Objective: During human pregnancy, the DNA methylation of placental tissue is highly relevant to the normal growth and development of the fetus; therefore, methylomic analysis of the placental tissue possesses high research and clinical value in prenatal testing and monitoring. Thus, our aim is to d...
| Publicado en: | Prenatal Diagnosis Vol. 38; no. 3; pp. 196 - 204 |
|---|---|
| Autores principales: | , , , , , |
| Formato: | equations & formulas pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Feb2018
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=128710984&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 128710984 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01973851 O9F jtl: Prenatal Diagnosis issn: 01973851 maglogo: Y pubinfo: dt: Feb2018 vid: 38 iid: 3 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 128710984 128710984 NLM29334402 128710984 10.1002/pd.5214 NLM29334402 128710984 ppf: 196 ppct: 8 formats: tig: atl: Noninvasive reconstruction of placental methylome from maternal plasma DNA: Potential for prenatal testing and monitoring. aug: au: Sun, Kun Lun, Fiona M. F. Leung, Tak Y. Chiu, Rossa W. K. Lo, Y. M. Dennis Sun, Hao affil: Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong sug: subj: DNA Methylation Placenta Metabolism Genomics Methods Prenatal Diagnosis Methods Human Pregnancy Female DNA Blood Algorithms Female ab: Objective: During human pregnancy, the DNA methylation of placental tissue is highly relevant to the normal growth and development of the fetus; therefore, methylomic analysis of the placental tissue possesses high research and clinical value in prenatal testing and monitoring. Thus, our aim is to develop an approach for reconstruction of the placental methylome, which should be completely noninvasive and achieve high accuracy and resolution.Results: We propose a novel size-based algorithm, FEtal MEthylome Reconstructor (FEMER), to noninvasively reconstruct the placental methylome by genomewide bisulfite sequencing and size-based analysis of maternal plasma DNA. By applying FEMER on a real clinical dataset, we demonstrate that FEMER achieves both high accuracy and resolution, thus provides a high-quality view of the placental methylome from maternal plasma DNA. FEtal MEthylome Reconstructor could also predict the DNA methylation profile of CpG islands with high accuracy, thus shows potential in monitoring of key genes involved in placental/fetal development. Source code and testing datasets for FEMER are available at http://sunlab.cpy.cuhk.edu.hk/FEMER/.Conclusion: FEtal MEthylome Reconstructor could enhance the noninvasive fetal/placental methylomic analysis and facilitate its application in prenatal testing and monitoring. pubtype: Academic Journal doctype: equations & formulas pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|