Prolonged persistence of IgM against dengue virus detected by commonly used commercial assays.
Background: Initial symptoms of dengue fever are non-specific, and thus definite diagnosis requires laboratory confirmation. Detection of IgM against dengue virus (DENV) has become widely used for dengue diagnosis. Understanding the persistence of anti-DENV IgM in subjects after acute infection is e...
| Published in: | BMC Infectious Diseases Vol. 18; no. 1; pp. 1 - 8 |
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| Main Authors: | , , , , , , , , , , , , , , , |
| Format: | research Journal Article |
| Published: |
BioMed Central
4/2/2018
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=128875540&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 128875540 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14712334 1CHU jtl: BMC Infectious Diseases issn: 14712334 maglogo: N pubinfo: dt: 4/2/2018 vid: 18 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 128875540 128875540 NLM29609533 128875540 10.1186/s12879-018-3058-0 NLM29609533 128875540 ppf: 1 ppct: 7 formats: tig: atl: Prolonged persistence of IgM against dengue virus detected by commonly used commercial assays. aug: au: Yu-Wen Chien Zi-Hu Liu Fan-Chen Tseng Tzu-Chuan Ho How-Ran Guo Nai-Ying Ko Wen-Chien Ko Guey Chuen Perng Chien, Yu-Wen Liu, Zi-Hu Tseng, Fan-Chen Ho, Tzu-Chuan Guo, How-Ran Ko, Nai-Ying Ko, Wen-Chien Perng, Guey Chuen affil: Department of Public Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan. sug: subj: Dengue Diagnosis Flaviviridae Immunology Immunoglobulins Blood Antibodies, Viral Blood Antibodies Analysis Aged Dengue Immunology Adult Young Adult Enzyme-Linked Immunosorbent Assay Methods Male Human Reagent Kits, Diagnostic Middle Age Female Taiwan Serologic Tests Business Prospective Studies Dengue Blood Proteins Immunology Validation Studies Comparative Studies Evaluation Research Multicenter Studies Aged: 65+ years Adult: 19-44 years Middle Aged: 45-64 years Male Female ab: Background: Initial symptoms of dengue fever are non-specific, and thus definite diagnosis requires laboratory confirmation. Detection of IgM against dengue virus (DENV) has become widely used for dengue diagnosis. Understanding the persistence of anti-DENV IgM in subjects after acute infection is essential in order to interpret test results correctly. Although the longevity of anti-DENV IgM has been vehemently investigated in symptomatic children, anti-DENV IgM persistence in adults and in asymptomatically infected people have seldom been reported.Methods: We prospectively investigated 44 adults with detectable anti-DENV IgM in a serosurvey conducted in the 2015 dengue epidemic in Tainan, Taiwan. Among subjects within the cohort, 17 were classified to be symptomatic and 27 were asymptomatic. The enzyme-linked immunosorbent assay (ELISA) from Standard Diagnostic (SD) and Focus Diagnostic were used to detect anti-DENV IgM for specimens collected initially, at 6 and 12 months. Regression analyses were used to estimate the duration of anti-DENV IgM fell below the detectable level. Rapid dengue tests from Standard Diagnostics had been widely adopted to detect anti-DENV IgM in Taiwan during the 2015 dengue outbreak. As such, collected specimens were also evaluated with the SD rapid dengue test in parallel.Results: Anti-DENV IgM was detectable in 70.5 and 46.2% of the 44 subjects at 6 months and 12 months by the SD ELISA, respectively, while 13.6 and 7.7%, respectively, by the Focus ELISA. There was no significant difference in anti-DENV IgM detection for the follow-up specimens between subjects with symptomatic and asymptomatic infections. The regression analysis estimated that anti-DENV IgM persistence fell to the undetectable level at 338.3 days (95% CI 279.7-446.9) by SD ELISA, while at 175.7 days (95% CI 121.9-221.1) by Focus ELISA. The detectable frequency of anti-DENV IgM by rapid tests was 86.4%, 68.2 and 35.9% at initial, 6 and 12 months, respectively.Conclusion: Anti-DENV IgM was found to persist much longer than previously thought, suggesting a necessity of re-evaluation of the use of anti-DENV IgM for both the diagnosis of dengue and serological surveillance, especially when large outbreaks have occurred in the preceding year. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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