Transcriptome comparison identifies potential biomarkers of spine and skull base chordomas.
Chordomas are rare, slowly growing, locally aggressive bone neoplasms that arise from embryonic remnants of the notochord, showing dual epithelial-mesenchymal differentiation. The high plasticity probably is the main reason for the high variety in phenotypes of chordoma, from its high heterogeneity...
| Publicado en: | Virchows Archiv: European Journal of Pathology Vol. 472; no. 3; pp. 489 - 498 |
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| Autores principales: | , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Mar2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=128908252&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 128908252 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09456317 O1Z jtl: Virchows Archiv: European Journal of Pathology issn: 09456317 maglogo: N pubinfo: dt: Mar2018 vid: 472 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 128908252 128908252 NLM28844110 128908252 10.1007/s00428-017-2224-x NLM28844110 128908252 ppf: 489 ppct: 9 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Transcriptome comparison identifies potential biomarkers of spine and skull base chordomas. aug: au: Bell, Achim H. DeMonte, Franco Raza, Shaan M. Rhines, Laurence D. Tatsui, Claudio E. Prieto, Victor G. Fuller, Gregory N. Bell, Diana affil: Pathology Department, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., 77030, Houston, TX, USA sug: subj: Spinal Neoplasms Skull Metabolism Neoplasms, Germ Cell and Embryonal Skull Neoplasms Gene Expression Profiling Skull Neoplasms Metabolism Spinal Neoplasms Metabolism Human Validation Studies Comparative Studies Evaluation Research Multicenter Studies ab: Chordomas are rare, slowly growing, locally aggressive bone neoplasms that arise from embryonic remnants of the notochord, showing dual epithelial-mesenchymal differentiation. The high plasticity probably is the main reason for the high variety in phenotypes of chordoma, from its high heterogeneity on a cellular level to its subtype variations depending on tissue location, with its potential to develop from an inactive quiescent form to an aggressive cancer with extreme adaptability and resistance to drugs and other treatments. Gene expression profiles of formalin-fixed, paraffin-embedded skull chordoma, spine chordoma, and normal tissue specimens were generated and compared. Using strict criteria, we identified 222 differentially expressed transcripts unique to skull base chordoma, 261 unique to spine chordoma, and 192 common to both chordoma subtypes. Further analysis of these three groups of transcripts allowed the selection of three subsets of highly differentially expressed genes as potential biomarkers, disease drivers, and therapeutic targets in both chordoma subtypes. Immunohistochemistry revealed LMX1A to be dominant in skull base chordoma, SALL3 to be unique to spine chordoma, and T to be common to both chordoma subtypes. In both chordoma subtypes, the genes with the highest expression were predominantly development-related genes, mostly transcription factors. Our findings indicate that these developmental genes play important oncogenic roles in chordoma, mainly causing high plasticity and resistance to therapy in both these cancer subtypes but also determining their differentiation status and proliferation activity, pointing to features expected of heterogeneous stem cell-like tissues with similarities to their notochord origins. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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