Tissue microarray is suitable for scientific biomarkers studies in endometrial cancer.
The aim of this study was to define the concordance between tissue microarrays (TMAs) of different sizes and whole slide for 15 different antibodies in endometrial cancer and study the use of TMAs in preoperative endometrial samples. Cores of preoperative and hysterectomy specimens of 14 endometrial...
| Publicado en: | Virchows Archiv: European Journal of Pathology Vol. 472; no. 3; pp. 407 - 414 |
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| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Mar2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=128908264&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 128908264 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09456317 O1Z jtl: Virchows Archiv: European Journal of Pathology issn: 09456317 maglogo: N pubinfo: dt: Mar2018 vid: 472 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 128908264 128908264 NLM29426961 128908264 10.1007/s00428-017-2289-6 NLM29426961 128908264 ppf: 407 ppct: 7 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Tissue microarray is suitable for scientific biomarkers studies in endometrial cancer. aug: au: Visser, Nicole C. M. van der Wurff, Anneke A. M. Pijnenborg, Johanna M. A. Massuger, Leon F. A. G. Bulten, Johan Nagtegaal, Iris D. affil: Department of Pathology, Radboud university medical center, P.O. Box 9101, 6500, Nijmegen, HB, the Netherlands sug: subj: Endometrial Neoplasms Pathology Tissue Array Analysis Methods Endometrial Neoplasms Metabolism Female Immunohistochemistry Methods Biopsy Methods Paraffin Embedding Methods Proteins Metabolism Human Female ab: The aim of this study was to define the concordance between tissue microarrays (TMAs) of different sizes and whole slide for 15 different antibodies in endometrial cancer and study the use of TMAs in preoperative endometrial samples. Cores of preoperative and hysterectomy specimens of 14 endometrial cancer and three atypical hyperplasia cases were collected in TMA blocks. Two 0.6-mm and two 2.0-mm cores were used from each sample. Different antibodies were tested in TMAs and compared with results of whole slides of hysterectomy. Tested antibodies were as follows: ER, PR, p53, Ki-67, MLH1, PMS2, MSH2, MSH6, ARID1A, stathmin, IMP3, L1CAM, PTEN, β-catenin, and p16. Seventeen cases with four cores per paraffin block (both 0.6 and 2.0 mm in duplicate) and 15 different antibodies resulted in a total of 1020 cores for both preoperative and hysterectomy specimen. Overall, 2.0-mm cores were more assessable for evaluation than 0.6-mm cores (96.0 versus 79.5%, p < 0.01). For most antibodies, a substantial to good agreement between hysterectomy TMA and whole slide was present, with lowest agreement for p16 and stathmin and perfect agreement for mismatch repair proteins. Preoperative TMAs showed for most antibodies moderate to perfect agreement with hysterectomy TMAs. In conclusion, 2.0-mm cores are the preferred size for immunohistochemical studies in endometrial cancer. For all tested antibodies, TMAs are a good alternative for whole slide analysis in scientific studies with large patient cohorts, even in preoperative endometrial samples. However, caution is required for interpretation of TMA results of p16 and stathmin. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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