Oligomeric states in sodium ion-dependent regulation of cyanobacterial histidine kinase-2.
Two-component signal transduction systems (TCSs) consist of sensor histidine kinases and response regulators. TCSs mediate adaptation to environmental changes in bacteria, plants, fungi and protists. Histidine kinase 2 (Hik2) is a sensor histidine kinase found in all known cyanobacteria and as chlor...
| Publicado en: | Protoplasma Vol. 255; no. 3; pp. 937 - 953 |
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| Autores principales: | , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
May2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=129132186&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 129132186 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0033183X 2CF jtl: Protoplasma issn: 0033183X maglogo: N pubinfo: dt: May2018 vid: 255 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 129132186 10.1007/s00709-017-1196-7 129132186 ppf: 937 ppct: 16 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Oligomeric states in sodium ion-dependent regulation of cyanobacterial histidine kinase-2. aug: au: Ibrahim, Iskander M. Wang, Liang Puthiyaveetil, Sujith Krauß, Norbert Nield, Jon Allen, John F. affil: Department of Biochemistry and Purdue Center for Plant Biology, Purdue University, West Lafayette, IN, USA sug: ab: Two-component signal transduction systems (TCSs) consist of sensor histidine kinases and response regulators. TCSs mediate adaptation to environmental changes in bacteria, plants, fungi and protists. Histidine kinase 2 (Hik2) is a sensor histidine kinase found in all known cyanobacteria and as chloroplast sensor kinase in eukaryotic algae and plants. Sodium ions have been shown to inhibit the autophosphorylation activity of Hik2 that precedes phosphoryl transfer to response regulators, but the mechanism of inhibition has not been determined. We report on the mechanism of Hik2 activation and inactivation probed by chemical cross-linking and size exclusion chromatography together with direct visualisation of the kinase using negative-stain transmission electron microscopy of single particles. We show that the functional form of Hik2 is a higher-order oligomer such as a hexamer or octamer. Increased NaCl concentration converts the active hexamer into an inactive tetramer. The action of NaCl appears to be confined to the Hik2 kinase domain. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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