Evaluation of urinary autophagy transcripts expression in diabetic kidney disease.
Background: We identified and validated novel urinary autophagy markers in diabetic kidney disease (DKD) based on bioinformatics analysis and clinical validation.Patients& Methods: We retrieved three novel autophagy genes related to DKD from public microarray databases, namely; microtubule-associate...
| Publicado en: | Journal of Diabetes & its Complications Vol. 31; no. 10; pp. 1491 - 1499 |
|---|---|
| Autores principales: | , , , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
Oct2017
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=129403538&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 129403538 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10568727 JIW jtl: Journal of Diabetes & its Complications issn: 10568727 maglogo: N pubinfo: dt: Oct2017 vid: 31 iid: 10 pid: 82545 pub: Elsevier B.V. place: Philadelphia, Pennsylvania artinfo: ui: 129403538 129403538 NLM28760651 129403538 10.1016/j.jdiacomp.2017.06.009 NLM28760651 129403538 ppf: 1491 ppct: 8 formats: tig: atl: Evaluation of urinary autophagy transcripts expression in diabetic kidney disease. aug: au: Matboli, Marwa Azazy, Ahmed E.M. Adel, Seham Bekhet, Miram M. Eissa, Sanaa affil: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Abbassia, P.O. Box 11381, Cairo, Egypt sug: subj: Membrane Proteins Metabolism Nerve Tissue Proteins Metabolism RNA Urine Protein-Tyrosine Kinases Metabolism Carrier Proteins Metabolism Diabetic Nephropathies Urine Biochemical Phenomena Diabetes Mellitus, Type 2 Complications Renal Insufficiency Diagnosis Diabetic Nephropathies Metabolism Kidney Physiopathology RNA Metabolism Carrier Proteins Autophagy Nerve Tissue Proteins Kidney Metabolism Membrane Proteins Aged Male Female Genes Bioinformatics Severity of Illness Indices Renal Insufficiency Complications Middle Age Renal Insufficiency Metabolism Prospective Studies Diabetic Nephropathies Diagnosis Human Albuminuria Etiology Diabetic Nephropathies Physiopathology Sensitivity and Specificity Protein-Tyrosine Kinases Renal Insufficiency Physiopathology Validation Studies Comparative Studies Evaluation Research Multicenter Studies Aged: 65+ years Middle Aged: 45-64 years Male Female ab: Background: We identified and validated novel urinary autophagy markers in diabetic kidney disease (DKD) based on bioinformatics analysis and clinical validation.Patients& Methods: We retrieved three novel autophagy genes related to DKD from public microarray databases, namely; microtubule-associated protein light chain (MAP1LC3A), WD Repeat Domain, Phosphoinositide Interacting 2 (WIPI2), and RB1-Inducible Coiled-Coil 1 (RB1CC1). Secondly we assessed the expression of the chosen autophagy transcript in urine sediment of 86 patients with DKD and 74 (age and sex matched) controls by reverse transcription quantitative real-time PCR.Results: The urinary expression levels of MAP1LC3A, WIPI, RB1CC1 were significantly lower in DKD than control group (P<0.001).The receiver-operating characteristic curve (ROC) analyses that each urinary autophagy transcript showed high sensitivity and specificity for distinguishing DKD from control (MAP1LC3A, 81.4% and 81.1%; WIPI, 74.4% and 67.6%, and RB1CC1, 81.4%,70.3%, respectively). Notably, a negative correlation was found between these autophagy markers, serum creatinine and urinary albumin creatinine ratio. The sensitivity and specificity of this urinary autophagy based panel reached 90.6% and 60% in diagnosis of DKD.Conclusion: We identified and validated a novel diagnostic urinary autophagy based panel with high sensitivity and moderate specificity representing a vital player in the pathogenesis of DKD. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|