Cloning and Expression of the Organophosphate Pesticide-Degrading α-β Hydrolase Gene in Plasmid pMK-07 to Confer Cross-Resistance to Antibiotics.
Pesticide residual persistence in agriculture soil selectively increases the pesticide-degrading population and transfers the pesticide-degrading gene to other populations, leading to cross-resistance to a wide range of antibiotics. The enzymes that degrade pesticides can also catabolize the antibio...
| Publicado en: | BioMed Research International Vol. 2018; pp. 1 - 14 |
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| Autores principales: | , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
5/16/2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=129637078&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 129637078 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 5/16/2018 vid: 2018 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 129637078 129637078 129637078 10.1155/2018/1535209 129637078 ppf: 1 ppct: 13 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Cloning and Expression of the Organophosphate Pesticide-Degrading α-β Hydrolase Gene in Plasmid pMK-07 to Confer Cross-Resistance to Antibiotics. aug: au: Rangasamy, Kirubakaran Athiappan, Murugan Devarajan, Natarajan Parray, Javid A. Shameem, Nowsheen Aruljothi, K. N. Hashem, Abeer Alqarawi, Abdulaziz A. Abd_Allah, Elsayed Fathi affil: Department of Microbiology, Periyar University, Salem, Tamil Nadu, India sug: subj: Genetic Techniques Gene Expression Organophosphorus Compounds Pesticides Hydrolases DNA Drug Resistance, Microbial Antibiotics Agriculture Human Catabolism Chromosomes Bacillus Sequence Analysis Enzymes Transferases Genetics Chloramphenicol Streptomycin Cefotaxime Ampicillin Tetracycline ab: Pesticide residual persistence in agriculture soil selectively increases the pesticide-degrading population and transfers the pesticide-degrading gene to other populations, leading to cross-resistance to a wide range of antibiotics. The enzymes that degrade pesticides can also catabolize the antibiotics by inducing changes in the gene or protein structure through induced mutations. The present work focuses on the pesticide-degrading bacteria isolated from an agricultural field that develop cross-resistance to antibiotics. This cross-resistance is developed through catabolic gene clusters present in an extrachromosomal plasmid. A larger plasmid (236.7 Kbp) isolated from<italic> Bacillus</italic> sp. was sequenced by next-generation sequencing, and important features such as <italic>α</italic>-<italic>β</italic> hydrolase, DNA topoisomerase, DNA polymerase III subunit beta, reverse transcriptase, plasmid replication rep X, recombination U, transposase, and S-formylglutathione hydrolase were found in this plasmid. Among these, the <italic>α</italic>-<italic>β</italic> hydrolase enzyme is known for the degradation of organophosphate pesticides. The cloning and expression of the <italic>α</italic>-<italic>β</italic> hydrolase gene imply nonspecific cleavage of antibiotics through a cross-resistance phenomenon in the host. The docking of <italic>α</italic>-<italic>β</italic> hydrolase with a spectrum of antibiotics showed a high G-score against chloramphenicol (−3.793), streptomycin (−2.865), cefotaxime (−5.885), ampicillin (−4.316), and tetracycline (−3.972). This study concludes that continuous exposure to pesticide residues may lead to the emergence of multidrug-resistant strains among the wild microbial flora. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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