HSP90: A Novel Target Gene of miRNA-628-3p in A549 Cells.
Lung cancer is one of the leading causes of cancer-related death in the world. MicroRNA- (miR-) 628-3p plays critical roles in many cancers, including lung cancer. We investigated how miR-628-3p affected migration and apoptosis in A549 cells. We used bioinformatics algorithms to predict the miR-628-...
| Publicado en: | BioMed Research International Vol. 2018; pp. 1 - 11 |
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| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
5/20/2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=129691440&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 129691440 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 5/20/2018 vid: 2018 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 129691440 129691440 129691440 10.1155/2018/4149707 129691440 ppf: 1 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: HSP90: A Novel Target Gene of miRNA-628-3p in A549 Cells. aug: au: Pan, Jieli Jiang, Fusheng Zhou, Jia Wu, Dehong Sheng, Zhenhua Li, Meiya affil: Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China sug: subj: Heat-Shock Proteins MicroRNA Cell Line, Tumor Cell Movement Apoptosis Human Bioinformatics Algorithms Lung Neoplasms Oxidoreductases Biological Assay Blotting, Western Polymerase Chain Reaction Biochemical Phenomena Gene Expression Transcription Factors ab: Lung cancer is one of the leading causes of cancer-related death in the world. MicroRNA- (miR-) 628-3p plays critical roles in many cancers, including lung cancer. We investigated how miR-628-3p affected migration and apoptosis in A549 cells. We used bioinformatics algorithms to predict the miR-628-3p target gene to study the molecular mechanism by which miR-628-3p contributes to lung cancer. Then, we used the luciferase reporter assay to identify whether heat shock protein 90a<italic> (HSP90)</italic> is a direct target of miR-628-3p. Western blotting and quantitative real-time PCR showed that miR-628-3p downregulated HSP90a protein expression via a posttranscriptional mechanism. We confirm that miR-628-3p promotes apoptosis and inhibits migration in A549 cells by negatively regulating<italic> HSP90</italic>. Our results may reveal a novel strategy for lung cancer treatment. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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