Detection of early neoplasia in Barrett's esophagus using lectin-based near-infrared imaging: an ex vivo study on human tissue.
Background and Study Aims: Endoscopic surveillance for Barrett's esophagus (BE) is limited by long procedure times and sampling error. Near-infrared (NIR) fluorescence imaging minimizes tissue autofluorescence and optical scattering. We assessed the feasibility of a topically applied NIR dye-labeled...
| Publicado en: | Endoscopy Vol. 50; no. 6; pp. 618 - 626 |
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| Autores principales: | , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Georg Thieme Verlag Stuttgart
2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=129852738&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 129852738 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0013726X NH1 jtl: Endoscopy issn: 0013726X maglogo: N pubinfo: dt: 2018 vid: 50 iid: 6 pid: 4536 pub: Georg Thieme Verlag Stuttgart artinfo: ui: 129852738 129852738 NLM29342490 10.1055/s-0043-124080 NLM29342490 129852738 ppf: 618 ppct: 8 formats: tig: atl: Detection of early neoplasia in Barrett's esophagus using lectin-based near-infrared imaging: an ex vivo study on human tissue. aug: au: Neves, André A. Di Pietro, Massimiliano Brindle, Kevin M. Waterhouse, Dale J. Bohndiek, Sarah E. O'Donovan, Maria Fitzgerald, Rebecca C. affil: Cancer Research UK Cambridge Institute, Li Ka-Shing Centre, Cambridge, UK. sug: subj: Esophageal Neoplasms Diagnostic Imaging Methods Esophagus Pathology Proteins Analysis Barrett Esophagus Molecular Imaging Methods Esophageal Neoplasms Pathology Hyperplasia Pathology Light Aged, 80 and Over Male Sensitivity and Specificity Aged Middle Age Pilot Studies Barrett Esophagus Pathology Hyperplasia Barrett Esophagus Surgery Esophageal Neoplasms Surgery Female Biopsy Staining and Labeling Arthritis Impact Measurement Scales Aged, 80 & over Aged: 65+ years Middle Aged: 45-64 years Male Female ab: Background and Study Aims: Endoscopic surveillance for Barrett's esophagus (BE) is limited by long procedure times and sampling error. Near-infrared (NIR) fluorescence imaging minimizes tissue autofluorescence and optical scattering. We assessed the feasibility of a topically applied NIR dye-labeled lectin for the detection of early neoplasia in BE in an ex vivo setting.Methods: Consecutive patients undergoing endoscopic mucosal resection (EMR) for BE-related early neoplasia were recruited. Freshly collected EMR specimens were sprayed at the bedside with fluorescent lectin and then imaged. Punch biopsies were collected from each EMR under NIR light guidance. We compared the fluorescence intensity from dysplastic and nondysplastic areas within EMRs and from punch biopsies with different histological grades.Results: 29 EMR specimens were included from 17 patients. A significantly lower fluorescence was found for dysplastic regions across whole EMR specimens (P < 0.001). We found a 41 % reduction in the fluorescence of dysplastic compared to nondysplastic punch biopsies (P < 0.001), with a sensitivity and specificity for dysplasia detection of 80 % and 82.9 %, respectively.Conclusion: Lectin-based NIR imaging can differentiate dysplastic from nondysplastic Barrett's mucosa ex vivo. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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