Bone Response to Titanium Implants Coated with Double- or Single-Stranded DNA.

We aimed to evaluate in vivo bone response and in vitro apatite formation to titanium (Ti) implants, coated with double-stranded DNA (DNA-d) or single-stranded DNA (DNA-s), and to compare the influence in different structure of DNA, double strand and single strand on bone response and apatite format...

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Publicado en:BioMed Research International Vol. 2018; pp. 1 - 12
Autores principales: Miyamoto, Nagahiro, Yamachika, Rina, Sakurai, Toshitsugu, Hayakawa, Tohru, Hosoya, Noriyasu
Formato: equations & formulas pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 6/13/2018
Acceso en línea:Ver este registro en EBSCOhost
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      issn: 23146133
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      dt: 6/13/2018
      vid: 2018
      pid: 480
      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        130129735
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        10.1155/2018/9204391
        130129735
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      tig:
        atl: Bone Response to Titanium Implants Coated with Double- or Single-Stranded DNA.
      aug:
        au:
          Miyamoto, Nagahiro
          Yamachika, Rina
          Sakurai, Toshitsugu
          Hayakawa, Tohru
          Hosoya, Noriyasu
        affil: Department of Endodontology, Tsurumi University School of Dental Medicine, Yokohama, Japan
      sug:
        subj:
          Titanium
          Prostheses and Implants
          DNA
          Bone and Bones
          Animal Studies
          In Vivo Studies
          In Vitro Studies
          Hydroxyapatites
          Dental Implants
          Maxilla
          Rats
      ab: We aimed to evaluate in vivo bone response and in vitro apatite formation to titanium (Ti) implants, coated with double-stranded DNA (DNA-d) or single-stranded DNA (DNA-s), and to compare the influence in different structure of DNA, double strand and single strand on bone response and apatite formation. The bone responses to multilayered DNA-d/protamine or DNA-s/protamine coating implants were evaluated after implantation into the extracted sockets of rat maxillary molars. Apatite formation on either coating surface after immersion in simulated body fluid (SBF) was evaluated using the quartz crystal microbalance (QCM) method. DNA-d/protamine and DNA-s/protamine coatings produced more roughened and hydrophilic surfaces than untreated Ti. Animal experiments showed that higher bone-to-implant ratios were achieved 3 and 6 weeks after implantation using DNA-d/protamine and DNA-s/protamine coatings compared with Ti. QCM measurements revealed that each coating contributed to significant earlier apatite formation in SBF. We conclude that both DNA-d/protamine and DNA-s/protamine coatings enhanced early bone formation. We suggest that a DNA-multilayer coating is useful for the surface modification of a Ti implant.
      pubtype: Academic Journal
      doctype:
        equations & formulas
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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