Forsythiaside inhibits bacterial adhesion on titanium alloy and attenuates Ti-induced activation of nuclear factor-κB signaling-mediated macrophage inflammation.
Background: Inflammation and biofilm formation by Staphylococcus aureus (S. aureus) are common causes of periprosthetic infection and loosening. Recently, we identified that forsythiaside is bacteriostatic for S. aureus and methicillin-resistant S. aureus (MRSA). The purpose of the present study was...
| Publicado en: | Journal of Orthopaedic Surgery & Research Vol. 13; no. 1; pp. 1 - 11 |
|---|---|
| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
BioMed Central
6/5/2018
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=130238021&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 130238021 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1749799X 38N9 jtl: Journal of Orthopaedic Surgery & Research issn: 1749799X maglogo: N pubinfo: dt: 6/5/2018 vid: 13 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 130238021 130238021 130238021 10.1186/s13018-018-0834-x 130238021 ppf: 1 ppct: 10 formats: tig: atl: Forsythiaside inhibits bacterial adhesion on titanium alloy and attenuates Ti-induced activation of nuclear factor-κB signaling-mediated macrophage inflammation. aug: au: Haifeng Li Dongmei Tang Chao Qi Xia Zhao Guangchao Wang Yi Zhang Tengbo Yu affil: Department of Sports Medicine, The Affiliated Hospital of Qingdao University, No. 16 Jiangsu Road, Qingdao 266003, China sug: subj: Plant Extracts Pharmacodynamics Bacterial Physiology Drug Effects NF-kappa B Drug Effects Signal Transduction Drug Effects Macrophages Drug Effects Inflammation Prevention and Control Biofilms Drug Effects Human In Vitro Studies Microscopy Methods Microscopy, Electron, Scanning Reverse Transcriptase Polymerase Chain Reaction Methods Blotting, Western Colony Count, Microbial Gene Expression Drug Effects Cytokines Drug Effects Staphylococcus Aureus Drug Effects Methicillin-Resistant Staphylococcus Aureus Drug Effects Joint Prosthesis Orthopedic Prosthesis Titanium Adverse Effects ab: Background: Inflammation and biofilm formation by Staphylococcus aureus (S. aureus) are common causes of periprosthetic infection and loosening. Recently, we identified that forsythiaside is bacteriostatic for S. aureus and methicillin-resistant S. aureus (MRSA). The purpose of the present study was to examine the effect of forsythiaside on S. aureus and MRSA adhesion and biofilm formation on the surface of titanium alloy, which is a popular material for orthopedic joint prostheses. Methods: Two strains of S. aureus and MRSA were used for in vitro experiments. The spread plate method, confocal laser scanning microscopy (CLSM), and scanning electron microscopy (SEM) were used to characterize antimicrobial activity of forsythiaside. Real-time polymerase chain reaction (RT-PCR) and western blotting were used to investigate the inhibitory level of forsythiaside required for titanium-associated inflammation. Results: Direct colony counting showed that 16 μg/mL forsythiaside significantly inhibited S. aureus and MRSA adhesion on titanium alloy discs in 2 h. CLSM and SEM showed that higher concentrations (> 30 mg/mL) of forsythiaside effectively inhibited the adhesion of S. aureus and MRSA on the surface of the titanium disc in 24 h. Forsythiaside was capable of attenuating Ti-induced activation of nuclear factor-κB signaling, targeting IxB kinase-α (IKKα) kinases of macrophages, and influencing the expression of NF-κB downstream cytokines. Conclusions: These observations suggest that forsythiaside is a potential agent for the treatment of Ti implant- associated infection and inflammation. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|