α-Tocopherol influences glycaemic control and <italic>miR-9-3</italic> DNA methylation in overweight and obese women under an energy-restricted diet: a randomized, double-blind, exploratory, controlled clinical trial.
Background: Excess weight is a strong risk factor for the development of dysglycaemia. It has been suggested that changes in the metabolism microRNAs, small non-coding RNAs that regulate gene expression, could precede late glycaemic changes. Vitamin E in turn may exert important functions in methyla...
| Publicado en: | Nutrition & Metabolism Vol. 15; no. 1 |
|---|---|
| Autores principales: | , , , , , , , , , , , , , , , |
| Formato: | research tables/charts randomized controlled trial Journal Article |
| Publicado: |
BioMed Central
7/11/2018
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=130637912&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 130637912 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17437075 1CYX jtl: Nutrition & Metabolism issn: 17437075 maglogo: N pubinfo: dt: 7/11/2018 vid: 15 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 130637912 130637912 130637912 10.1186/s12986-018-0286-7 130637912 ppct: 1 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: α-Tocopherol influences glycaemic control and <italic>miR-9-3</italic> DNA methylation in overweight and obese women under an energy-restricted diet: a randomized, double-blind, exploratory, controlled clinical trial. aug: au: Luna, Rafaella Cristhine Pordeus dos Santos Nunes, Mayara Karla Monteiro, Mussara Gomes Cavalcante Alves da Silva, Cássia Surama Oliveira do Nascimento, Rayner Anderson Ferreira Lima, Raquel Patrícia Ataíde Pimenta, Flávia Cristina Fernandes de Oliveira, Naila Francis Paulo Persuhn, Darlene Camati de Almeida, Aléssio Tony Cavalcanti da Silva Diniz, Alcides Pissetti, Cristina Wide Vianna, Rodrigo Pinheiro Toledo de Lima Ferreira, Flavia Emília Leite Rodrigues Gonçalves, Maria da Conceição de Carvalho Costa, Maria José affil: Postgraduate in Nutrition Sciences, Health Sciences Center Federal University of Paraíba (Universidade Federal da Paraíba) 58051-900 João Pessoa Paraíba Brazil sug: subj: Vitamin E Pharmacodynamics Glycemic Control Drug Effects DNA Methylation Drug Effects Obesity Diet Therapy Restricted Diet MicroRNA Drug Effects Human Randomized Controlled Trials Double-Blind Studies Exploratory Research Female Polymerase Chain Reaction Methods Anthropometry Fasting Blood Glucose Drug Effects Glycated Hemoglobin Drug Effects Insulin Blood Female ab: Background: Excess weight is a strong risk factor for the development of dysglycaemia. It has been suggested that changes in the metabolism microRNAs, small non-coding RNAs that regulate gene expression, could precede late glycaemic changes. Vitamin E in turn may exert important functions in methylation and gene expression processes. This study aimed to determine the effect of α-tocopherol on glycaemic variables and <italic>miR-9-1</italic> and <italic>miR-9-3</italic> promoter DNA methylation in overweight women. Methods: A randomized, double-blind, exploratory, placebo-controlled study was conducted in overweight and obese adult women (<italic>n</italic> = 44) who ingested synthetic vitamin E (all-rac-α-tocopherol), natural source vitamin E (RRR-rac-α-tocopherol) or placebo capsules and were followed up for a period of 8 weeks. Supplemented groups also received dietary guidance for an energy-restricted diet. An additional group that received no supplementation and did not follow an energy-restricted diet was also followed up. The intervention effect was evaluated by DNA methylation levels (quantitative real-time PCR assay) and anthropometric and biochemical variables (fasting plasma glucose, haemoglobin A1C, insulin, and vitamin E). Results: Increased methylation levels of the <italic>miR-9-3</italic> promoter region (<italic>P</italic> < 0.001) and reduced haemoglobin A1C (<italic>P</italic> < 0.05) were observed in the natural source vitamin E group after intervention. Increased fasting plasma glucose was observed in the synthetic vitamin E group, despite the significant reduction of anthropometric variables compared to the other groups. Conclusions: α-Tocopherol from natural sources increased methylation levels of the <italic>miR-9-3</italic> promoter region and reduced haemoglobin A1C in overweight women following an energy-restricted diet. These results provide novel information about the influence of vitamin E on DNA methylation. Trial registration: <ext-link>ClinicalTrials.gov</ext-link>, <ext-link>NCT02922491</ext-link>. Registered 4 October, 2016. pubtype: Academic Journal doctype: research tables/charts randomized controlled trial Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|