Anatomy, Functionality, and Neuronal Connectivity with Manganese Radiotracers for Positron Emission Tomography.
Purpose: Manganese ion has been extensively used as a magnetic resonance imaging (MRI) contrast agent in preclinical studies to assess tissue anatomy, function, and neuronal connectivity. Unfortunately, its use in human studies has been limited by cellular toxicity and the need to use a very low dos...
| Publicado en: | Molecular Imaging & Biology Vol. 20; no. 4; pp. 562 - 575 |
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| Autores principales: | , , , , , |
| Formato: | diagnostic images research Journal Article |
| Publicado: |
Springer Nature
Aug2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=130645755&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 130645755 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Aug2018 vid: 20 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 130645755 130645755 NLM29396750 130645755 10.1007/s11307-018-1162-6 NLM29396750 130645755 ppf: 562 ppct: 13 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Anatomy, Functionality, and Neuronal Connectivity with Manganese Radiotracers for Positron Emission Tomography. aug: au: Saar, Galit Millo, Corina M. Szajek, Lawrence P. Bacon, Jeff Herscovitch, Peter Koretsky, Alan P. affil: Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 20892, Bethesda, MD, USA sug: subj: Nervous System Tomography, Emission-Computed Nervous System Anatomy and Histology Radioisotopes Manganese Tomography, X-Ray Computed Primates Diagnostic Imaging Administration, Intranasal Glucose Metabolism Neural Pathways Manganese Administration and Dosage Radioisotopes Administration and Dosage Indicators and Reagents Rats Pancreas Magnetic Resonance Imaging Animal Studies ab: Purpose: Manganese ion has been extensively used as a magnetic resonance imaging (MRI) contrast agent in preclinical studies to assess tissue anatomy, function, and neuronal connectivity. Unfortunately, its use in human studies has been limited by cellular toxicity and the need to use a very low dose. The much higher sensitivity of positron emission tomography (PET) over MRI enables the use of lower concentrations of manganese, potentially expanding the methodology to humans.Procedures: PET tracers manganese-51 (Mn-51, t1/2 = 46 min) and manganese-52 (Mn-52, t1/2 = 5.6 days) were used in this study. The biodistribution of manganese in animals in the brain and other tissues was studied as well as the uptake in the pancreas after glucose stimulation as a functional assay. Finally, neuronal connectivity in the olfactory pathway following nasal administration of the divalent radioactive Mn-52 ([52Mn]Mn2+) was imaged.Results: PET imaging with the divalent radioactive Mn-51 ([51Mn]Mn2+) and [52Mn]Mn2+ in both rodents and monkeys demonstrates that the accumulation of activity in different organs is similar to that observed in rodent MRI studies following systemic administration. Furthermore, we demonstrated the ability of manganese to enter excitable cells. We followed activity-induced [51Mn]Mn2+ accumulation in the pancreas after glucose stimulation and showed that [52Mn]Mn2+ can be used to trace neuronal connections analogous to manganese-enhanced MRI neuronal tracing studies.Conclusions: The results were consistent with manganese-enhanced MRI studies, despite the much lower manganese concentration used for PET (100 mM Mn2+ for MRI compared to ~ 0.05 mM for PET). This indicates that uptake and transport mechanisms are comparable even at low PET doses. This helps establish the use of manganese-based radiotracers in both preclinical and clinical studies to assess anatomy, function, and connectivity. pubtype: Academic Journal doctype: diagnostic images research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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