A microfluidic device for motility and osmolality analysis of zebrafish sperm.
A microfluidic chip is described that facilitates research and quality control analysis of zebrafish sperm which, due to its miniscule (i.e., 2-5 μl) sample volume and short duration of motility (i.e., <1 min), present a challenge for traditional manual assessment methods. A micromixer molded in pol...
| Publicado en: | Biomedical Microdevices Vol. 20; no. 3; pp. 1 - 2 |
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| Autores principales: | , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Sep2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=131169360&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 131169360 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13872176 ODN jtl: Biomedical Microdevices issn: 13872176 maglogo: N pubinfo: dt: Sep2018 vid: 20 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 131169360 10.1007/s10544-018-0308-2 131169360 ppf: 1 ppct: 1 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: A microfluidic device for motility and osmolality analysis of zebrafish sperm. aug: au: Beckham, Jacob Alam, Faiz Omojola, Victor Scherr, Thomas Guitreau, Amy Melvin, Adam Park, Daniel S. Choi, Jin-Woo Tiersch, Terrence R. Todd Monroe, W. affil: Department of Biological & Agricultural Engineering, Louisiana State University and Agricultural Center, Baton Rouge, LA, USA sug: ab: A microfluidic chip is described that facilitates research and quality control analysis of zebrafish sperm which, due to its miniscule (i.e., 2-5 μl) sample volume and short duration of motility (i.e., <1 min), present a challenge for traditional manual assessment methods. A micromixer molded in polydimethylsiloxane (PDMS) bonded to a glass substrate was used to activate sperm samples by mixing with water, initiated by the user depressing a transfer pipette connected to the chip. Sample flow in the microfluidic viewing chamber was able to be halted within 1 s, allowing for rapid analysis of the sample using established computer-assisted sperm analysis (CASA) methods. Zebrafish sperm cell activation was consistent with manual hand mixing and yielded higher values of motility at earlier time points, as well as more subtle time-dependent trends in motility, than those processed by hand. Sperm activation curves, which indicate sample quality by evaluating percentage and duration of motility at various solution osmolalities, were generated with on-chip microfabricated gold floor electrodes interrogated by impedance spectroscopy. The magnitude of admittance was linearly proportional to osmolality and was not affected by the presence of sperm cells in the vicinity of the electrodes. This device represents a pivotal step in streamlining methods for consistent, rapid assessment of sperm quality for aquatic species. The capability to rapidly activate sperm and consistently measure motility with CASA using the microfluidic device described herein will help improve the reproducibility of studies on sperm and assist development of germplasm repositories. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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