CD4+ and CD8+ T-Cell-Specific DNA Cytosine Methylation Differences Associated With Obesity.
Objective: Lifestyle factors associated with obesity may alter epigenome-regulated gene expression. Most studies examining epigenetic changes in obesity have analyzed DNA 5´-methylcytosine (5mC) in whole blood, representing a weighted average of several distantly related and regulated leukocyte clas...
| Publicado en: | Obesity (19307381) Vol. 26; no. 8; pp. 1312 - 1322 |
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| Autores principales: | , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Wiley-Blackwell
Aug2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=131393979&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 131393979 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 19307381 GSO jtl: Obesity (19307381) issn: 19307381 maglogo: Y pubinfo: dt: Aug2018 vid: 26 iid: 8 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 131393979 131393979 NLM29956501 131393979 10.1002/oby.22225 NLM29956501 131393979 ppf: 1312 ppct: 10 formats: tig: atl: CD4+ and CD8+ T-Cell-Specific DNA Cytosine Methylation Differences Associated With Obesity. aug: au: Hohos, Natalie M. Smith, Alicia K. Kilaru, Varun Park, Hea Jin Hausman, Dorothy B. Bailey, Lynn B. Lewis, Richard D. Phillips, Bradley G. Meagher, Richard B. affil: Department of Foods and Nutrition, University of Georgia, Athens, Georgia, USA sug: subj: T Lymphocytes Metabolism Obesity Obesity Immunology DNA Methylation Physiology Leukocytes Metabolism Genes Adipose Tissue Metabolism Cells, Cultured Genes Physiology Young Adult Heterocyclic Compounds Female Adult Human Case Control Studies Pilot Studies Body Weight Adolescence Obesity Metabolism Validation Studies Comparative Studies Evaluation Research Multicenter Studies Adult: 19-44 years Adolescent: 13-18 years Female ab: Objective: Lifestyle factors associated with obesity may alter epigenome-regulated gene expression. Most studies examining epigenetic changes in obesity have analyzed DNA 5´-methylcytosine (5mC) in whole blood, representing a weighted average of several distantly related and regulated leukocyte classes. To examine leukocyte-specific differences associated with obesity, a pilot study examining 5mC in three distinct leukocyte types isolated from peripheral blood of women with normal weight and obesity was conducted.Methods: CD4+ T cells, CD8+ T cells, and CD16+ neutrophils were reiteratively isolated from blood, and 5mC levels were measured across >450,000 CG sites.Results: Nineteen CG sites were differentially methylated between women with obesity and with normal weight in CD4+ cells, 16 CG sites in CD8+ cells, and 0 CG sites in CD16+ neutrophils (q < 0.05). There were no common differentially methylated sites between the T-cell types. The amount of visceral adipose tissue was strongly associated with the methylation level of 79 CG sites in CD4+ cells, including 4 CG sites in CLSTN1's promoter, which, this study shows, may regulate its expression.Conclusions: The methylomes of various leukocytes respond differently to obesity and levels of visceral adipose tissue. Highly significant differentially methylated sites in CD4+ and CD8+ cells in women with obesity that have apparent biological relevance to obesity were identified. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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