Varying miR-193b-3p Expression Patterns in Breast Cancer Cell Lines Indicates Its Potential for Cancer Management Strategies.
Background: Metastasis-associated miRNA (metastamiR) harbors a great potential to confine metastasis as the most lethal aspect of cancer. miR-193b-3p is an anti-metastatic miRNA, whose expression significantly decreases in metastatic breast cancer cells. Objectives: In the present study, the express...
| Publicado en: | International Journal of Cancer Management Vol. 11; no. 8; pp. 1 - 10 |
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| Autores principales: | , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Medical Journals Commission of the Ministry of Health & Medical Education
Aug2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=131463589&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 131463589 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 25384422 L6AI jtl: International Journal of Cancer Management issn: 25384422 maglogo: N pubinfo: dt: Aug2018 vid: 11 iid: 8 pid: 66482 pub: Medical Journals Commission of the Ministry of Health & Medical Education artinfo: ui: 131463589 131463589 131463589 10.5812/ijcm.63540 131463589 ppf: 1 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Varying miR-193b-3p Expression Patterns in Breast Cancer Cell Lines Indicates Its Potential for Cancer Management Strategies. aug: au: Hashemi, Zahra Sadat Moghadam, Mehdi Forouzandeh Sadroddiny, Esmaeil affil: Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran sug: subj: Breast Neoplasms Therapy Cell Line, Tumor Analysis MicroRNA Analysis Genetic Techniques Methods Disease Management Human Neoplasm Metastasis Polymerase Chain Reaction Methods Cell Movement Biological Assay ab: Background: Metastasis-associated miRNA (metastamiR) harbors a great potential to confine metastasis as the most lethal aspect of cancer. miR-193b-3p is an anti-metastatic miRNA, whose expression significantly decreases in metastatic breast cancer cells. Objectives: In the present study, the expression patterns of different cell-lines were investigated, following an effective miR transfection strategy. Methods: Double-stranded oligo of mature miR-193b-3p, miR-negative, and miR-LacZ were designed and cloned into pcDNA6.2gw/EmGFP plasmid. Calculating the population doubling time (PDT), non-tumorigenic MCF-10A, tumorigenic but non-metastatic MCF-7, and metastatic MDA-MB231 were transfected by Lipofectamin2000 and Express-In. The expressions of miR-193b-3p and miR-191 have been quantified by Real-time PCR 48 hours after transfection. Scratch, Transwell migration, and Matrigel invasion assays have been carried out to assess the migration and invasion levels of the cell-lines. Results: The PDT (21.27 ± 0.43, 28.18 ± 0.34, and 35.83 ± 0.44 hours) and miR-193b-3p relative expression before transfection (100, 42 and 8) were measured for MCF-10A, MCF-7, and MDA-MB231 cell-lines, respectively. Better transfection results were obtained based on nano-liposome method. The expression of the miR-193b-3p was increased 32, 19 and, 65 fold-change. The rate of invasion in metastatic cells was 6.6 fold-higher in comparison to MCF-7 cells. Conclusions: Higher expression rate of the miRNA is anticipated to occur, following miR-mimic transfection. However, the observed differential patterns of miRNA increase in the context of different cell-lines, indicating the involvement of more complicated cellular pathways. Scrutinizing these cellular mechanisms could open new horizons in cancer therapy and management strategies. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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