Validation of digital microscopy in the histopathological diagnoses of oral diseases.
Whole slide imaging (WSI) systems are being increasingly used in educational and professional settings, highlighting the value of digital microscopy and favouring its acceptance for use in primary diagnosis. There has been a reluctance to introduce diagnostic applications due to a lack of validation...
| Publicado en: | Virchows Archiv: European Journal of Pathology Vol. 473; no. 3; pp. 321 - 328 |
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| Autores principales: | , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Springer Nature
Sep2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=131498402&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 131498402 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09456317 O1Z jtl: Virchows Archiv: European Journal of Pathology issn: 09456317 maglogo: N pubinfo: dt: Sep2018 vid: 473 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 131498402 131498402 NLM29858684 131498402 10.1007/s00428-018-2382-5 NLM29858684 131498402 ppf: 321 ppct: 7 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Validation of digital microscopy in the histopathological diagnoses of oral diseases. aug: au: Araújo, Anna Luíza Damaceno Amaral-Silva, Gleyson Kleber Palmier, Natália Rangel Lopes, Marcio Ajudarte de Almeida, Oslei Paes Vargas, Pablo Agustin Santos-Silva, Alan Roger Fonseca, Felipe Paiva Speight, Paul M. affil: Oral Diagnosis Department, Semiology and Oral Pathology Areas, Piracicaba Dental School, University of Campinas (UNICAMP), Av. Limeira, 901, Bairro Areão, 13414-903, Piracicaba, São Paulo, Brazil sug: subj: Mouth Diseases Microscopy Methods Human Mouth Diseases Pathology Cross Sectional Studies Retrospective Design Observer Bias Funding Source ab: Whole slide imaging (WSI) systems are being increasingly used in educational and professional settings, highlighting the value of digital microscopy and favouring its acceptance for use in primary diagnosis. There has been a reluctance to introduce diagnostic applications due to a lack of validation and regulation of these devices. This study aims to provide information regarding the performance of WSI and to validate it for use in the diagnosis of oral diseases, using the intraobserver variability as the primary form of analysis. Seventy (n = 70) H&E-stained glass slides of oral biopsies were scanned using the Aperio Digital Pathology System at a magnification of × 20. Two experienced oral pathologists blindly analysed all H&E-stained sections with a conventional light microscope (CLM) and, after 3-month washout, with WSI. Clinical information was provided along with the cases in both analyses. The intraobserver agreement between CLM and WSI was 97% (κ = 0.9) for both pathologists. The majority of preferred diagnoses were by CLM. Both pathologists had the same discordances in different cases. Challenging cases and cases with insufficient quantity of tissue for analyses were considered the main reasons for disagreement rather than the diagnostic methods. Median time taken to make a diagnosis was higher only in CLM for one pathologist. Time outliers occurred in discordant cases and in other difficult cases. This study provides evidence of a high performance of WSI for diagnostic purposes in clinical practice, routine pathology and primary diagnosis in the field of oral pathology. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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