Renal Graft Fibrosis and Inflammation Quantification by an Automated Fourier-Transform Infrared Imaging Technique.
Renal interstitial fibrosis and interstitial active inflammation are the main histologic features of renal allograft biopsy specimens. Fibrosis is currently assessed by semiquantitative subjective analysis, and color image analysis has been developed to improve the reliability and repeatability of t...
| Published in: | Journal of the American Society of Nephrology (JASN) Vol. 27; no. 8; pp. 2382 - 2392 |
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| Main Authors: | , , , , , |
| Format: | research Journal Article |
| Published: |
Lippincott Williams & Wilkins
Aug2016
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=131893498&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 131893498 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10466673 1X25 jtl: Journal of the American Society of Nephrology (JASN) issn: 10466673 maglogo: N pubinfo: dt: Aug2016 vid: 27 iid: 8 pid: 433 pub: Lippincott Williams & Wilkins place: Baltimore, Maryland artinfo: ui: 131893498 131893498 NLM26683669 131893498 10.1681/ASN.2015050601 NLM26683669 131893498 ppf: 2382 ppct: 10 formats: tig: atl: Renal Graft Fibrosis and Inflammation Quantification by an Automated Fourier-Transform Infrared Imaging Technique. aug: au: Vuiblet, Vincent Fere, Michael Gobinet, Cyril Birembaut, Philippe Piot, Olivier Rieu, Philippe affil: Matrice Extracellulaire et Dynamique Cellulaire Unit, Centre National pour la Recherche Scientifique, Unité Mixte de Recherche 7369, and Nephrology and Renal Transplantation Department and Biopathology Laboratory, Centre Hospitalier et Universitaire de Reims, Reims, France sug: subj: Postoperative Complications Pathology Inflammation Pathology Kidney Pathology Kidney Diseases Pathology Kidney Transplantation Female Middle Age Fibrosis Retrospective Design Spectrophotometry, Infrared Male Human Middle Aged: 45-64 years Female Male ab: Renal interstitial fibrosis and interstitial active inflammation are the main histologic features of renal allograft biopsy specimens. Fibrosis is currently assessed by semiquantitative subjective analysis, and color image analysis has been developed to improve the reliability and repeatability of this evaluation. However, these techniques fail to distinguish fibrosis from constitutive collagen or active inflammation. We developed an automatic, reproducible Fourier-transform infrared (FTIR) imaging-based technique for simultaneous quantification of fibrosis and inflammation in renal allograft biopsy specimens. We generated and validated a classification model using 49 renal biopsy specimens and subsequently tested the robustness of this classification algorithm on 166 renal grafts. Finally, we explored the clinical relevance of fibrosis quantification using FTIR imaging by comparing results with renal function at 3 months after transplantation (M3) and the variation of renal function between M3 and M12. We showed excellent robustness for fibrosis and inflammation classification, with >90% of renal biopsy specimens adequately classified by FTIR imaging. Finally, fibrosis quantification by FTIR imaging correlated with renal function at M3, and the variation in fibrosis between M3 and M12 correlated well with the variation in renal function over the same period. This study shows that FTIR-based analysis of renal graft biopsy specimens is a reproducible and reliable label-free technique for quantifying fibrosis and active inflammation. This technique seems to be more relevant than digital image analysis and promising for both research studies and routine clinical practice. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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