High-dose vitamin D3 supplementation decreases the number of colonic CD103+ dendritic cells in healthy subjects.
Purpose: Vitamin D may induce tolerance in the intestinal immune system and has been shown to regulate the phenotype of tolerogenic intestinal dendritic cells (DCs) in vitro. It is unknown whether vitamin D supplementation affects human intestinal DCs in vivo, and we aimed to investigate the tolerab...
| Publicado en: | European Journal of Nutrition Vol. 57; no. 7; pp. 2607 - 2620 |
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| Autores principales: | , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Springer Nature
Oct2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=131975661&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 131975661 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Oct2018 vid: 57 iid: 7 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 131975661 131975661 131975661 10.1007/s00394-017-1531-y 131975661 ppf: 2607 ppct: 13 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: High-dose vitamin D3 supplementation decreases the number of colonic CD103+ dendritic cells in healthy subjects. aug: au: Bak, Nina Friis Bendix, M. Hald, S. Reinert, L. Magnusson, M. K. Agnholt, J. affil: Department of Hepatology and Gastroenterology, Aarhus University Hospital, Noerrebrogade 44 Building 1C Ground Floor, Aarhus C, Denmark sug: subj: Cholecalciferol Administration and Dosage Dietary Supplementation Dendritic Cells Drug Effects Colon Drug Effects Cholecalciferol Pharmacodynamics Administration, Oral Biopsy Methods Human Endoscopy Mucous Membrane Antigens, Surface Flow Cytometry Polymerase Chain Reaction Methods T Lymphocytes RNA, Messenger Gene Expression Tumor Necrosis Factor Interleukins Factor Analysis Apoptosis Cytokines Intestinal Mucosa Colonic Diseases Diagnosis ab: Purpose: Vitamin D may induce tolerance in the intestinal immune system and has been shown to regulate the phenotype of tolerogenic intestinal dendritic cells (DCs) in vitro. It is unknown whether vitamin D supplementation affects human intestinal DCs in vivo, and we aimed to investigate the tolerability and effect on intestinal CD103+DCs of high-dose vitamin D3 treatment in healthy subjects.Methods: Ten healthy subjects received a total of 480,000 IU oral vitamin D3 over 15 days and colonic biopsies were obtained before and after intervention by endoscopy. Lamina propria mononuclear cells (LPMCs) were isolated from the biopsies, stained with DC surface markers and analysed with flow cytometry. Snap-frozen biopsies were analysed with qPCR for DC and regulatory T cell-related genes.Results: No hypercalcemia or other adverse events occurred in the test subjects. Vitamin D decreased the number of CD103+ DCs among LPMCs (p = 0.006). Furthermore, vitamin D induced mRNA expression of TGF-β (p = 0.048), TNF-α (p = 0.006) and PD-L1 (p = 0.02) and tended to induce IL-10 expression (p = 0.06). Multivariate factor analysis discriminated between pre- and post-vitamin D supplementation with a combined increased qPCR expression of PD1, PD-L1, TGF-β, IL-10, CD80, CD86, FOXP3, NFATc2 and cathelicidin.Conclusion: High-dose vitamin D supplementation is well tolerated by healthy subjects and has a direct effect on the CD103+ DCs, local cytokine and surface marker mRNA expression in the colonic mucosa, suggestive of a shift towards a more tolerogenic milieu. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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