Designing a novel ELISA method based on CagA, NapA recombinant antigens to increase sensitivity and specificity of Helicobacter pylori whole cell antigen detection.
Aim: In this research, we designed a direct Enzyme Linked Immunoassay method to detect Helicobacter pylori antigens in stool specimens. Background: Helicobacter pylori infection as the worldwide problem is related to many gastrointestinal disorders such as gastritis, gastric cancer, non-ulcer diseas...
| Publicado en: | Gastroenterology & Hepatology from Bed to Bench Vol. 11; no. 4; pp. 333 - 343 |
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| Autores principales: | , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Gastroenterology & Hepatology from Bed to Bench
Autumn2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=132602030&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 132602030 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 20082258 B2GW jtl: Gastroenterology & Hepatology from Bed to Bench issn: 20082258 maglogo: N pubinfo: dt: Autumn2018 vid: 11 iid: 4 pid: 61561 pub: Gastroenterology & Hepatology from Bed to Bench artinfo: ui: 132602030 132602030 132602030 132602030 ppf: 333 ppct: 10 formats: tig: atl: Designing a novel ELISA method based on CagA, NapA recombinant antigens to increase sensitivity and specificity of Helicobacter pylori whole cell antigen detection. aug: au: Kamarehei, Farideh Khabiri, Alireza Saidijam, Massoud Soleimani, Meysam Alikhani, Mohammad Yousef affil: Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran sug: subj: Helicobacter Infections Diagnosis Enzyme-Linked Immunosorbent Assay Methods Recombinant Proteins Diagnostic Use Helicobacter Pylori Immunology Human Sensitivity and Specificity Program Development Helicobacter Infections Complications Qualitative Studies Quantitative Studies Histocytochemistry Urease Blood Staining and Labeling Confidence Intervals Helicobacter Infections Prevention and Control ab: Aim: In this research, we designed a direct Enzyme Linked Immunoassay method to detect Helicobacter pylori antigens in stool specimens. Background: Helicobacter pylori infection as the worldwide problem is related to many gastrointestinal disorders such as gastritis, gastric cancer, non-ulcer disease, peptic ulcer disease and duodenal ulcer. Methods: We produced and purified recombinant CagA and NapA antigens in Escherichia coli and extracted their antibodies from a panel of positive sera specimens. We designed a novel enzyme linked immunoassay direct method in combination with the whole cell for the qualitative and quantitative detection of Helicobacter pylori antigens in human stool. Assay performance was evaluated by histopathology staining and urease activity. Results: The sensitivity and specificity of assay was determined as 91.7 [95% confidence interval: 89.3-95.6%] and 93.1% [95% CI: 91.2-96.4%], respectively. Novel ELISA exhibits enhanced sensitivity and specificity of Helicobacter pylori detection in comparison with another commercially available kit. Conclusion: Combination of the recombinant antigens and whole cell of Helicobacter pylori in immunoassay designing is a new approach about early diagnosis, treatment and fallowing up of the Helicobacter pylori infected patients, especially in peptic cancer cases. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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