Dual staining for p16/Ki67 is a more specific test than cytology for triage of HPV-positive women.

Globally, cervical cancer (CC) screening is moving from cytology-based to HPV screening or a combination of both (co-testing). Most HPV-positive women clear the virus and do not develop relevant disease. Additional triage approaches are needed to reduce unnecessary colposcopy referrals. The p16/Ki67...

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Publicado en:Virchows Archiv: European Journal of Pathology Vol. 473; no. 5; pp. 599 - 607
Autores principales: Areán-Cuns, Carolina, Mercado-Gutiérrez, Maria, Paniello-Alastruey, Irene, Mallor-Giménez, Fermín, Córdoba-Iturriagagoitia, Alicia, Lozano-Escario, Maria, Santamaria-Martínez, Mercedes
Formato: Journal Article
Publicado: Springer Nature Nov2018
Acceso en línea:Ver este registro en EBSCOhost
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        atl: Dual staining for p16/Ki67 is a more specific test than cytology for triage of HPV-positive women.
      aug:
        au:
          Areán-Cuns, Carolina
          Mercado-Gutiérrez, Maria
          Paniello-Alastruey, Irene
          Mallor-Giménez, Fermín
          Córdoba-Iturriagagoitia, Alicia
          Lozano-Escario, Maria
          Santamaria-Martínez, Mercedes
        affil: Department of Pathology, Complejo Hospitalario de Navarra, Calle Irunlarrea 3, Pabellón F, Servicio de Anatomía Patológica, 31008, Pamplona, Spain
      sug:
        subj:
          Proteins Metabolism
          Triage Methods
          Tumor Markers, Biological Metabolism
          Papillomavirus Infections Complications
          Cervix Neoplasms Diagnosis
          Carcinoma, Squamous Cell Diagnosis
          Middle Age
          Adolescence
          Carcinoma, Squamous Cell
          Aged
          Adult
          Carcinoma, Squamous Cell Metabolism
          Aged, 80 and Over
          Prospective Studies
          Young Adult
          Sensitivity and Specificity
          Cytodiagnosis
          Cervix Neoplasms
          Female
          Cervix Neoplasms Metabolism
          Middle Aged: 45-64 years
          Adolescent: 13-18 years
          Aged: 65+ years
          Adult: 19-44 years
          Aged, 80 & over
          Female
      ab: Globally, cervical cancer (CC) screening is moving from cytology-based to HPV screening or a combination of both (co-testing). Most HPV-positive women clear the virus and do not develop relevant disease. Additional triage approaches are needed to reduce unnecessary colposcopy referrals. The p16/Ki67 dual stain cytology test (DSCT) is one of the most promising, but it has not (yet) been included as a recommendation in European guidelines. Previous studies in Spain on this issue are lacking. We studied the performance of p16/Ki67 DSCT for the triage of HPV-positive women in Navarra to detect precursor lesions (PLs) and CC compared to cytology only. We selected 1865 HPV-positive women with p16/Ki67 DSCT results and 304 women with an available biopsy result. Sensitivity, specificity and predictive values of the p16/Ki67 DSCT to detect underlying PLs and CC compared to cytology were calculated, using the biopsy as the gold standard. Cytology and p16/Ki67 DSCT showed similar sensitivity (99.0% vs. 98.0%), but cytology had significantly lower specificity (6.9 vs. 39.1%). Of the CIN2+/HPV+ women, triage using cytology only would have resulted in 40.2% true PLs and CC, while using p16/Ki67 DSCT this was 98.0% qualifying the women for colposcopy referral. Our results show that p16/Ki67 DSCT detects more than twice as many true PLs and CC than cytology only in this population. Thus, this test can be considered as an important additional tool in HPV testing-based screening strategies, to avoid unnecessary colposcopy referrals and to reduce health care costs.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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