Investigation of Frequency of Mycoplasma hominis and Biological Parameters in Semen Sample of Men Referred to Qom Jihad Daneshgahi Infertility Treatment Center in 2016 (Iran).
Background and Objectives: Mycoplasmas hominis is one of the important causes of bacterial infections of the reproductive system, which through changes in the volume, pH, motility, morphology, and sperm count in men, can lead to reduction in the level of fertility or infertility and sterility. In th...
| Publicado en: | Qom University of Medical Sciences Journal Vol. 12; no. 4; pp. 81 - 89 |
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| Autores principales: | , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Qom University of Medical Sciences
Jun2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=133428162&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 133428162 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17357799 8P2V jtl: Qom University of Medical Sciences Journal issn: 17357799 maglogo: N pubinfo: dt: Jun2018 vid: 12 iid: 4 pid: 50887 pub: Qom University of Medical Sciences artinfo: ui: 133428162 133428162 133428162 133428162 ppf: 81 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Investigation of Frequency of Mycoplasma hominis and Biological Parameters in Semen Sample of Men Referred to Qom Jihad Daneshgahi Infertility Treatment Center in 2016 (Iran). aug: au: Asgari, Ali Nazari, Razieh Hossein Razavian, Seyed Mohammad affil: Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran sug: subj: Health Facilities Iran Infertility Semen Analysis Mycoplasma Analysis Biological Markers Analysis Referral and Consultation Polymerase Chain Reaction Human Iran Male Descriptive Research Analytic Research Practice Guidelines Genomics DNA RNA, Messenger T-Tests Descriptive Statistics Bacterial Infections Sperm Motility Male ab: Background and Objectives: Mycoplasmas hominis is one of the important causes of bacterial infections of the reproductive system, which through changes in the volume, pH, motility, morphology, and sperm count in men, can lead to reduction in the level of fertility or infertility and sterility. In this research, the frequency of Mycoplasma hominis, was assessed by PCR method, and biological parameters, were investigated in the semen samples of infertile men referred to Qom jihad daneshgahi infertility treatment center. Methods: In this descriptive-analytic study, a total of 187 semen samples were collected from infertile men referred to Qom jihad daneshgahi infertility treatment center from April to September 2016. Semen analysis was performed according to the guideline of World Health Organization. After the extraction of genomic DNA, detection of Mycoplasma hominis, was performed by PCR method using 16S rRNA specific primers. Data were analyzed using dependent and independent t-test. Results: Among 187 semen samples studied by PCR, 71 samples (38%), were positive for Mycoplasma hominis. In the assessment of parameters, there was a significant association between bacterial infection and C class mean difference (in situ sperm motility) (p=0.008). Conclusion: The results of the present study showed that high percentage of infertile men are infected with Mycoplasma hominis, which may lead to pelvic inflammatory diseases, urethritis, and infertility. PCR method is a rapid and efficient technique for detection of mycoplasma, which can be useful in the control of sexual transmitted diseases. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: Persian refInfo: holdings: @attributes: islocal: N |
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