Gossypol Promotes Bone Formation in Ovariectomy-Induced Osteoporosis through Regulating Cell Apoptosis.
Osteoporosis is among the most common forms of age-related diseases, especially for females, which has been a grave public health problem. Drug therapies have shown promising outcomes to promote bone formation and bone density. This study identified a novel potential drug, gossypol, for the treatmen...
| Publicado en: | BioMed Research International pp. 1 - 10 |
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| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
12/13/2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=133533936&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 133533936 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 12/13/2018 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 133533936 133533936 133533936 10.1155/2018/3635485 133533936 ppf: 1 ppct: 9 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Gossypol Promotes Bone Formation in Ovariectomy-Induced Osteoporosis through Regulating Cell Apoptosis. aug: au: Liang, Jinqian Chen, Chong Liu, Hongzhe Liu, Xiangyang Li, Zheng Hu, Jianhua Zhao, Hong affil: Department of Orthorpaedic Surgery, Peking Union Medical College Hospital, Beijing 100730, China sug: subj: Contraceptive Agents, Male Pharmacodynamics Oophorectomy Adverse Effects Osteoporosis Drug Therapy Apoptosis Drug Effects Osteoblasts Drug Effects Osteogenesis Drug Effects Animal Studies Mice In Vitro Studies Osteocalcin Drug Effects Receptors, Immunologic Drug Effects Membrane Proteins Drug Effects Tomography, X-Ray Computed Methods Cortical Bone Drug Effects Cell Viability Drug Effects Bone Density Drug Effects Calcium Binding Proteins Drug Effects Genetic Techniques Methods Blotting, Western Signal Transduction Drug Effects Wnt Proteins Drug Effects Cytoskeletal Proteins Drug Effects ab: Osteoporosis is among the most common forms of age-related diseases, especially for females, which has been a grave public health problem. Drug therapies have shown promising outcomes to promote bone formation and bone density. This study identified a novel potential drug, gossypol, for the treatment of osteoporosis. Treatments of ovariectomy-induced osteoporosis mice with gossypol significantly increased serum osteocalcin and osteoprotegerin (OPG) levels; meanwhile they decreased serum RANKL levels. Microcomputed tomography (microCT) analysis showed that treatment of gossypol improved bone density and strength and decreased bone postyield displacement for both medullar and cortical bones. In vitro experiments also showed that gossypol increased cell viability in a time- and dose-dependent manner. Furthermore, incubation of the osteoblast MC3T3-E1 cells with gossypol inhibited cell apoptosis through intrinsic apoptotic pathway as evidenced by the Annexin V/PI assay, TUNEL assay, biochemical analysis, and western blot assays. Moreover, the classical Wnt/β-catenin signaling pathway was found to be regulated by gossypol treatments. Inhibition of Wnt/β-catenin signaling reversed the prevention effects of gossypol in osteoporosis. Our findings provided novel clues for the treatment of osteoporosis in clinic. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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