Genotyping and antifungal susceptibility profile of clinical Cryptococcus neoformans species complex...5th Iranian Congress in Medical Mycology, Tehran, Iran, Dec 4-6, 2018.
Introduction: Cryptococcus neoformans and Cryptococcus gattii are the major causative agents of human cryptococcosis. Cryptococcosis recognized as an acute, subacute or chronic infectious mycotic zoonosis of world-wide significance and have a wide range of appearances such as meningitis, meningoence...
| Publicado en: | Current Medical Mycology Vol. 4; pp. 42 - 44 |
|---|---|
| Autores principales: | , , , , , , |
| Formato: | abstract proceedings research Journal Article |
| Publicado: |
Mazandaran University of Medical Sciences
2018 Supplement 1
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=133659395&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 133659395 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 24233439 JKN0 jtl: Current Medical Mycology issn: 24233439 maglogo: N pubinfo: dt: 2018 Supplement 1 vid: 4 pid: 45683 pub: Mazandaran University of Medical Sciences artinfo: ui: 133659395 133659395 133659395 10.18502/cmm.4.S1.2018.179 133659395 ppf: 42 ppct: 2 formats: tig: atl: Genotyping and antifungal susceptibility profile of clinical Cryptococcus neoformans species complex...5th Iranian Congress in Medical Mycology, Tehran, Iran, Dec 4-6, 2018. aug: au: Bandlizadeh, Zainab shokohi, Tahereh Badali, Hamid Mardani, Masoud Khodavaisy, Sadegh Afshari, Setareh Agha Kuchak Ahmadikia, Kazem affil: Student Research Committee, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran sug: subj: Genotype Cryptococcus Antifungal Agents Congresses and Conferences Iran Iran ab: Introduction: Cryptococcus neoformans and Cryptococcus gattii are the major causative agents of human cryptococcosis. Cryptococcosis recognized as an acute, subacute or chronic infectious mycotic zoonosis of world-wide significance and have a wide range of appearances such as meningitis, meningoencephalitis, pneumonia, osteomyelitis, abscesses in various internal organs and ocular disorders. Meningitis is the most frequent manifestation of cryptococcosis. The aims of this comprehensive study were to determine the molecular types of clinical Cryptococcus neoformans isolates by URA5-RFLP; to survey for the presence of the two mating types: a and a; and to determine the antifungal susceptibility profile of the clinical isolates. Materials and Methods: 220 clinical samples from 212 patients with suspected cryptococcosis were collected from April 2016 to June 2018. The clinical specimens included: cerebrospinal fluid (CSF), broncho alveolar lavage (BAL), spinal cord abscess, aspirated vesicles, sputum, brain biopsy and paraffin block of brain abscess. PCR-RFLP of the URA5 gene, mating type determination, in vitro susceptibility test to antifungal drugs alone and in combination (checkerboard technique) were performed. Result: out of 220 samples, 14 samples were positive. The prevalence of cryptococcosis was 5.7% (12/220) and all patients affected to meningitis. The most prevalent molecular type was VNI (85.7%) followed by VNII (14.3%). The lowest MIC values ranged from 0.031 - 0.125µg/ml) were observed for voriconazole. The checkerboard methods of antifungal combinations of amphotericin B with 5- fluorocytosine revealed synergistic interaction against 6 (43%) isolates, indifferent interactions against 8 (57%) isolates. The combination amphotericin B and fluconazole resulted synergistic interaction against 4 (28.5%) isolates, antagonism against 3(21.5%) isolates and indifferent against 7(50%) isolates. The combination of 5-FC with fluconazole yielded synergistic interaction against 3 (21.5%) isolates and indifferent 11(78.5%) isolates. Conclusion: The majority of the isolates, 87.5% (n=12), were VNI (var. grubii, serotype A), which accords with the fact that this variety causes most human cryptococcal infections worldwide. pubtype: Academic Journal doctype: abstract proceedings research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|