The Removal of HBV in Plasma by Extracorporeal Immunoadsorption from Plasma: A Potential Therapy of Hepatitis B Patients.
Objective. To establish a novel HBV specific immunoadsorbent for the removing of HBV particles. Methods. The anti-HBsAg monoclonal antibody was immobilized on sepharose beads to produce a sepharose anti-HBs column. Then the immunoadsorbent was evaluated and characterized by scanning electron microsc...
| Publicado en: | BioMed Research International pp. 1 - 8 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
12/23/2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=133710580&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 133710580 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 12/23/2018 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 133710580 133710580 133710580 10.1155/2018/1269063 133710580 ppf: 1 ppct: 7 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: The Removal of HBV in Plasma by Extracorporeal Immunoadsorption from Plasma: A Potential Therapy of Hepatitis B Patients. aug: au: Han, Zhenwei Lu, Xuan Tang, Yiping Yang, Yuanyuan Liu, Qiuchen Cheng, Pengfei Zhou, Li Wang, Yefu affil: State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan 430072, China sug: subj: Hepatitis B Blood Immunosorbent Techniques Methods Hepatitis B Therapy Molecular Structure Human Antibodies, Monoclonal Blood Polysaccharides Microscopy, Electron, Scanning Sensitivity and Specificity Decontamination, Hazardous Materials Polymerase Chain Reaction Enzyme-Linked Immunosorbent Assay Antigens, Viral Blood ab: Objective. To establish a novel HBV specific immunoadsorbent for the removing of HBV particles. Methods. The anti-HBsAg monoclonal antibody was immobilized on sepharose beads to produce a sepharose anti-HBs column. Then the immunoadsorbent was evaluated and characterized by scanning electron microscopy. In addition, time-dependent effects of the eradication capacity of anti-HBsAg functionalized sepharose beads against HBV were investigated. Results. Proposed immunoadsorbents exhibited a favorable biocompatibility as well as specificity. With the optimized recycle time, the decontamination performance of HBV particles and quantity of HBsAg were assessed either by real-time quantitative PCR or ELISA, which showed that the immunoadsorbent could remove approximately 90% of the HBV and 90% of the HBsAg from human plasma samples. Conclusions. All these results indicated that the novel immunoadsorbent could effectively remove HBV particles and likely serve as a novel therapy option or at least supplementary for the treatment regimen of HBV. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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