Evaluation of Helicobacter pylori Infection and Clarithromycin Resistance in Strains From Symptomatic Colombian Children.
The aim of the study was to determine the current prevalence of Helicobacter pylori in symptomatic Colombian children and evaluate the presence of mutations associated with clarithromycin resistance. Biopsies from 133 children were analyzed. The gastric fragment was used for urease test and reused f...
| Publicado en: | Journal of Pediatric Gastroenterology & Nutrition Vol. 67; no. 5; pp. 601 - 605 |
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| Autores principales: | , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Wiley-Blackwell
Nov2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=134052206&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 134052206 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 02772116 N1F jtl: Journal of Pediatric Gastroenterology & Nutrition issn: 02772116 maglogo: N pubinfo: dt: Nov2018 vid: 67 iid: 5 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 134052206 134052206 NLM29727437 134052206 10.1097/MPG.0000000000002016 NLM29727437 134052206 ppf: 601 ppct: 4 formats: tig: atl: Evaluation of Helicobacter pylori Infection and Clarithromycin Resistance in Strains From Symptomatic Colombian Children. aug: au: Rosero, Yuliet L. Arévalo-Jaimes, Betsy V. Delgado, María Pilar Vera-Chamorro, José F. García, Daniella Ramírez, Andrea Rodriguez-Urrego, Paula A. Álvarez, Johanna Jaramillo, Carlos A. affil: Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia sug: subj: Clarithromycin Immunology Antibiotics Immunology Helicobacter Infections Microbiology Helicobacter Pylori Drug Resistance, Microbial Stomach Microbiology Female Adolescence Microbial Culture and Sensitivity Tests Stomach Pathology Human Prevalence Biopsy Polymerase Chain Reaction Genotype Child, Preschool Helicobacter Pylori Drug Effects Helicobacter Infections Epidemiology Child Male Mutation Helicobacter Infections Drug Therapy Cross Sectional Studies Colombia Validation Studies Comparative Studies Evaluation Research Multicenter Studies Adolescent: 13-18 years Child, Preschool: 2-5 years Child: 6-12 years Female Male ab: The aim of the study was to determine the current prevalence of Helicobacter pylori in symptomatic Colombian children and evaluate the presence of mutations associated with clarithromycin resistance. Biopsies from 133 children were analyzed. The gastric fragment was used for urease test and reused for polymerase chain reaction sequencing of the 23SrDNA gene. Mutations were detected by bioinformatic analysis. Polymerase chain reaction sequencing established that H pylori infection was present in 47% of patients. Bioinformatics analysis of the 62 positive sequences for 23SrDNA revealed that 92% exhibited a genotype susceptible to clarithromycin, whereas the remaining strains (8%) showed mutations associated with clarithromycin resistance. The low rate of resistance to clarithromycin (8%) suggests that conventional treatment methods are an appropriate choice for children. Recycling a biopsy that is normally discarded reduces the risks associated with the procedure. The 23SrDNA gene amplification could be used for a dual purpose: detection of H pylori and determination of susceptibility to clarithromycin. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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