Efficacy of a Simple Static Cleaning Procedure for SPTFF.
Continuous bioproduction processes have recently seen a flood of development activity. A key technology that can help achieve a continuous production flow is single-pass tangential flow filtration (SPTFF); a continuous tangential flow filtration (TFF) operation with a simple, straight-through flow p...
| Publicado en: | BioPharm International Vol. 32; no. 1; pp. 29 - 36 |
|---|---|
| Autores principales: | , , |
| Formato: | Journal Article |
| Publicado: |
MJH Life Sciences
Jan2019
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=134257357&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 134257357 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1542166X 5NE jtl: BioPharm International issn: 1542166X maglogo: N pubinfo: dt: Jan2019 vid: 32 iid: 1 pid: 54670 pub: MJH Life Sciences place: Cranbury, New Jersey artinfo: ui: 134257357 134257357 ppf: 29 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Efficacy of a Simple Static Cleaning Procedure for SPTFF. aug: au: BISSCHOP, TORSTEN CHAN, KAREN LUTZ, HERB affil: Biomanufacturing engineer consultant, MilliporeSigma sug: ab: Continuous bioproduction processes have recently seen a flood of development activity. A key technology that can help achieve a continuous production flow is single-pass tangential flow filtration (SPTFF); a continuous tangential flow filtration (TFF) operation with a simple, straight-through flow path without a recirculation loop. SPTFF is performed at lower feed flow rates than those of batch TFF operations. SPTFF is used to directly link different unit operations (i.e., to alter in-line buffer composition and product concentration or to increase the final concentration at high yield). However, there are limitations in the available options and flow rates used for cleaning the filters. Yet, the high value of TFF devices makes the assessment of reuse by cleaning economically interesting. This article highlights a case study in which a static cleaning methodology was applied for SPTFF. To maintain the simplicity of the straight-through flow path, a cleaning procedure was assessed using a combination of flushing and static hold steps without recirculation. The cleaning procedure efficacy and its effect on process performance over multiple cycles of a monoclonal antibody process were evaluated. pubtype: Trade Publication doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|