New Gene Markers of Angiogenesis and Blood Vessels Development in Porcine Ovarian Granulosa Cells during Short-Term Primary Culture In Vitro.
The physiological processes that drive the development of ovarian follicle, as well as the process of oogenesis, are quite well known. Granulosa cells are major players in this occurrence, being the somatic element of the female gamete development. They participate directly in the processes of oogen...
| Published in: | BioMed Research International pp. 1 - 13 |
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| Main Authors: | , , , , , , , , , , , , , , , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Wiley-Blackwell
1/30/2019
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=134372690&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 134372690 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 1/30/2019 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 134372690 134372690 134372690 10.1155/2019/6545210 134372690 ppf: 1 ppct: 12 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: New Gene Markers of Angiogenesis and Blood Vessels Development in Porcine Ovarian Granulosa Cells during Short-Term Primary Culture In Vitro. aug: au: Chermuła, Błażej Brązert, Maciej Iżycki, Dariusz Ciesiółka, Sylwia Kranc, Wiesława Celichowski, Piotr Ożegowska, Katarzyna Nawrocki, Mariusz J. Jankowski, Maurycy Jeseta, Michal Antosik, Paweł Bukowska, Dorota Skowroński, Mariusz T. Brussow, Klaus P. Bruska, Małgorzata Pawelczyk, Leszek Zabel, Maciej Nowicki, Michał Kempisty, Bartosz affil: Department of Infertility and Reproductive Endocrinology, Poznan University of Medical Sciences, Poznan, Poland sug: subj: Neovascularization, Physiologic Granulosa Cells Cell Culture Techniques Genetic Markers Animal Studies Swine In Vitro Studies RNA Oligonucleotide Array Sequence Analysis P-Value Chemokines Receptors, Cell Surface Reverse Transcriptase Polymerase Chain Reaction Methods Intracellular Signaling Peptides and Proteins Somatomedins ab: The physiological processes that drive the development of ovarian follicle, as well as the process of oogenesis, are quite well known. Granulosa cells are major players in this occurrence, being the somatic element of the female gamete development. They participate directly in the processes of oogenesis, building the cumulus-oocyte complex surrounding the ovum. In addition to that, they have a further impact on the reproductive processes, being a place of steroid sex hormone synthesis and secretion. It is known that the follicle development creates a major need for angiogenesis and blood vessel development in the ovary. In this study, we use novel molecular approaches to analyze markers of these processes in porcine granulosa cultured primarily in vitro. The cells were recovered from mature sus scrofa specimen after slaughter. They were then subjected to enzymatic digestion and culture primarily for a short term. The RNA was extracted from cultures in specific time periods (0h, 24h, 48h, 96h, and 144h) and analyzed using expression microarrays. The genes that exhibited fold change bigger than |2|, and adjusted p-value lower than 0.05, were considered differentially expressed. From these, we have chosen the members of "angiogenesis," "blood vessel development," "blood vessel morphogenesis," "cardiovascular system development," and "vasculature development" for further selection. CCL2, FGFR2, SFRP2, PDPN, DCN, CAV1, CHI3L1, ITGB3, FN1, and LOX which are upregulated, as well as CXCL10, NEBL, IHH, TGFBR3, SCUBE1, IGF1, EDNRA, RHOB, PPARD, and SLITRK5 genes whose expression is downregulated through the time of culture, were chosen as the potential markers, as their expression varied the most during the time of culture. The fold changes were further validated with RT-qPCR. The genes were described, with special attention to their possible function in GCs during culture. The results broaden the general knowledge about GC's in vitro molecular processes and might serve as a point of reference for further in vivo and clinical studies. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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