Ex vivo orbital volumetry using stereology and CT imaging: A comparison with manual planimetry.
Objectives: To evaluate the applicability of stereology and planimetry in orbital volume measurements using computed tomography (CT) and to compare the results between the two measurements.Methods: Experimental study using sheep craniums for CT imaging. Water filling measurements were performed, as...
| Publicado en: | European Radiology Vol. 29; no. 3; pp. 1365 - 1375 |
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| Autores principales: | , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Mar2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=134415314&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 134415314 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09387994 NPH jtl: European Radiology issn: 09387994 maglogo: N pubinfo: dt: Mar2019 vid: 29 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 134415314 134415314 NLM30135979 134415314 10.1007/s00330-018-5691-9 NLM30135979 134415314 ppf: 1365 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Ex vivo orbital volumetry using stereology and CT imaging: A comparison with manual planimetry. aug: au: Bontzos, Georgios Mazonakis, Michael Papadaki, Efrosini Maris, Thomas G. Blazaki, Styliani Drakonaki, Eleni E. Detorakis, Efstathios T. affil: Department of Ophthalmology, University Hospital of Heraklion, 71110, Stavrakia, Heraklion, Crete, Greece sug: subj: Tomography, X-Ray Computed Methods Orbit Sheep ROC Curve Animal Studies Models, Biological Reproducibility of Results Validation Studies Comparative Studies Evaluation Research Multicenter Studies ab: Objectives: To evaluate the applicability of stereology and planimetry in orbital volume measurements using computed tomography (CT) and to compare the results between the two measurements.Methods: Experimental study using sheep craniums for CT imaging. Water filling measurements were performed, as the validation technique. Quantification techniques were also evaluated in five human subjects. To examine the proportion of agreement among measurements, we tested intra- and inter-observer agreement.Results: For stereology customization, a 1/8 systematic sampling scheme was considered as optimal; this resulted in a low coefficient of error (2.59 %) and low measurement time (1.9 mins). In sheep craniums, mean volume measured by water displacement, planimetry and stereology was 17.81 ± 0.59 cm3, 17.87 ± 0.68 cm3 and 17.54 ± 0.49 cm3, respectively. Total volumes, obtained by stereology, were highly correlated with the water-filling method (r=0.893; p = 0.001) and a paired t-test showed significant difference between methods (t=3.047; p = 0.014). Planimetry results displayed a high correlation with the water-filling method (r=0.957; p ≈ 0.001) but no statistically significant difference was found (p = 0.154). Mean difference using planimetry and stereology was 0.332 ± 0.322 cm3. In human subjects, using stereology, the estimated volume ranged between 18.57 cm3 and 19.27 cm3, and the mean orbital volume was 19.05 ± 0.50 cm3 with CE=3.75 ± 0.16 %. Mean measure time was 2.1 ± 0.1 mins.Conclusions: Stereological measurements were superior to manual planimetry in terms of user effort and time spent. Stereology sampling of 1/8 was successfully applied in human subjects and yielded a strong correlation with manual planimetry.Key Points: • Stereology can be applied to measure the orbital volume using computed tomography. • Stereological measurements display high correlation with gold standard planimetry and combine low coefficient of error (2.59%) with low measurement time (1.9 min). • Stereology is superior in terms of user effort and time spent. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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