Effects of Cervical Rotatory Manipulation (CRM) on Carotid Atherosclerosis Plaque in Vulnerability: A Histological and Immunohistochemical Study Using Animal Model.

Background. The safety of cervical rotatory manipulation (CRM) is still controversial, especially in patients with carotid artery atherosclerosis (CAS). The study aimed to investigate the effects of CRM on carotid plaques in vulnerability. Methods. 50 rabbits were randomly divided into four groups:...

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Publicado en:BioMed Research International pp. 1 - 11
Autores principales: Qi, Ji, Ping, Ruiyue, Zhang, Shaoqun, Xu, Yanxiao, Wu, Kai, Li, Yikai
Formato: diagnostic images pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 2/4/2019
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 2/4/2019
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1155/2019/3793840
        134461244
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        atl: Effects of Cervical Rotatory Manipulation (CRM) on Carotid Atherosclerosis Plaque in Vulnerability: A Histological and Immunohistochemical Study Using Animal Model.
      aug:
        au:
          Qi, Ji
          Ping, Ruiyue
          Zhang, Shaoqun
          Xu, Yanxiao
          Wu, Kai
          Li, Yikai
        affil: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China
      sug:
        subj:
          Carotid Artery Diseases Therapy
          Immunohistochemistry
          Manipulation, Orthopedic Methods
          Vulnerability
          Histological Techniques
          Animal Studies
          Rabbits
          Dietary Fats
          C-Reactive Protein Blood
          Staining and Labeling Methods
          Carotid Stenosis
          Gene Expression
          Treatment Outcomes
      ab: Background. The safety of cervical rotatory manipulation (CRM) is still controversial, especially in patients with carotid artery atherosclerosis (CAS). The study aimed to investigate the effects of CRM on carotid plaques in vulnerability. Methods. 50 rabbits were randomly divided into four groups: model rabbits with CRM [CAS-CRM (n=15)]; model rabbits without CRM [CAS (n=15)]; normal rabbits with CRM [Normal-CRM (n=10)]; and Blank-control group (n=10). CAS disease models were induced by carotid artery balloon injury combined with a high-fat diet for 12 weeks. Then, CRM technique was performed in CAS-CRM and Normal-CRM groups for 3 weeks. In the end, determination of serum level of hs-CRP and Lp-PLA2, histological analysis under HE and Masson trichromic staining, and immunohistochemical analysis with CD34 and CD68 antibody were completed in order. Results. Carotid stenosis rates on successful model rabbits ranged from 70% to 98%. The CAS-CRM group had an increased level of hs-CRP (P<0.05), in comparison with the CAS group, whereas effects were not significant between the Normal-CRM group and Blank-control group. In comparison with the CAS group, the positive expression of CD34 and CD68 in the CAS-CRM group increased significantly (P<0.05). Conclusion. CRM therapy may increase the vulnerability of carotid plaque in rabbits with severe CAS.
      pubtype: Academic Journal
      doctype:
        diagnostic images
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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