Tumor Formation of Adult Stem Cell Transplants in Rodent Arthritic Joints.
Purpose: While imaging matrix-associated stem cell transplants aimed for cartilage repair in a rodent arthritis model, we noticed that some transplants formed locally destructive tumors. The purpose of this study was to determine the cause for this tumor formation in order to avoid this complication...
| Publicado en: | Molecular Imaging & Biology Vol. 21; no. 1; pp. 95 - 105 |
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| Autores principales: | , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Feb2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=134786712&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 134786712 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Feb2019 vid: 21 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 134786712 134786712 NLM29869062 10.1007/s11307-018-1218-7 NLM29869062 134786712 ppf: 95 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Tumor Formation of Adult Stem Cell Transplants in Rodent Arthritic Joints. aug: au: Chapelin, Fanny Khurana, Aman Moneeb, Mohammad Gray Hazard, Florette K. Chan, Chun Fai Ray Nejadnik, Hossein Gratzinger, Dita Messing, Solomon Erdmann, Jason Gaur, Amitabh Daldrup-Link, Heike E. affil: Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University, 725 Welch Rd, Rm 1665, 94305-5654, Stanford, CA, USA sug: subj: Stem Cells Transplantation Cell Transformation, Neoplastic Pathology Arthritis Therapy Neoplasms, Fibrous Tissue Etiology Chondrosarcoma Etiology Femur Pathology Rats Rodents Joints Pathology Bone Neoplasms Pathology Chondrosarcoma Pathology Neoplasms, Fibrous Tissue Diagnosis Animals Stem Cells Pathology Bone Neoplasms Etiology Arthritis Pathology Neoplasms, Fibrous Tissue Pathology Arthritis Diagnosis Cells, Cultured Joints Chondrosarcoma Diagnosis Femur Male Magnetic Resonance Imaging Bone Neoplasms Diagnosis Arthritis Impact Measurement Scales Male ab: Purpose: While imaging matrix-associated stem cell transplants aimed for cartilage repair in a rodent arthritis model, we noticed that some transplants formed locally destructive tumors. The purpose of this study was to determine the cause for this tumor formation in order to avoid this complication for future transplants.Procedures: Adipose-derived stem cells (ADSC) isolated from subcutaneous adipose tissue were implanted into 24 osteochondral defects of the distal femur in ten athymic rats and two immunocompetent control rats. All transplants underwent serial magnetic resonance imaging (MRI) up to 6 weeks post-transplantation to monitor joint defect repair. Nine transplants showed an increasing size over time that caused local bone destruction (group 1), while 11 transplants in athymic rats (group 2) and 4 transplants in immunocompetent rats did not. We compared the ADSC implant size and growth rate on MR images, macroscopic features, histopathologic features, surface markers, and karyotypes of these presumed neoplastic transplants with non-neoplastic ADSC transplants.Results: Implants in group 1 showed a significantly increased two-dimensional area at week 2 (p = 0.0092), 4 (p = 0.003), and 6 (p = 0.0205) compared to week 0, as determined by MRI. Histopathological correlations confirmed neoplastic features in group 1 with significantly increased size, cellularity, mitoses, and cytological atypia compared to group 2. Six transplants in group 1 were identified as malignant chondrosarcomas and three transplants as fibromyxoid sarcomas. Transplants in group 2 and immunocompetent controls exhibited normal cartilage features. Both groups showed a normal ADSC phenotype; however, neoplastic ADSC demonstrated a mixed population of diploid and tetraploid cells without genetic imbalance.Conclusions: ADSC transplants can form tumors in vivo. Preventive actions to avoid in vivo tumor formations may include karyotyping of culture-expanded ADSC before transplantation. In addition, serial imaging of ADSC transplants in vivo may enable early detection of abnormally proliferating cell transplants. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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