Less Vertebral Bone Mass after Treatment with Macitentan in Mice: A Pilot Study.
Purpose. Blood vessels and skeleton interact together. Endothelin-1 is a potent vasoconstrictor and also has an effect on bone metabolism. The dual antagonist to both endothelin-1 type A and B receptors, Macitentan, has been approved for clinical management of pulmonary arterial hypertension while l...
| Publicado en: | BioMed Research International pp. 1 - 7 |
|---|---|
| Autores principales: | , , , , , , , , |
| Formato: | diagnostic images pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
2/19/2019
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=134793317&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 134793317 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2/19/2019 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 134793317 134793317 134793317 10.1155/2019/2075968 134793317 ppf: 1 ppct: 6 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Less Vertebral Bone Mass after Treatment with Macitentan in Mice: A Pilot Study. aug: au: Liu, Zhong-Yu Au, Man-Ting He, Tian-Wei Yang, Bu Liu, Bin Zhang, Liang-Ming Luo, Chun-Xiao Rong, Li-Min Wen, Chun-Yi affil: Department of Spine Surgery, Institute of Drug Clinical Trial for Orthopedic Diseases, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China sug: subj: Receptors, Cell Surface Antagonists and Inhibitors Bone Density Drug Effects Receptors, Cell Surface Pharmacodynamics Spine Drug Effects Endothelins Pharmacodynamics Endothelins Antagonists and Inhibitors Animal Studies Mice Molecular Structure Pilot Studies Administration, Intraperitoneal Receptors, Cell Surface Administration and Dosage Endothelins Blood Enzyme-Linked Immunosorbent Assay Tomography, X-Ray Computed Methods Histological Techniques Methods Osteoblasts Cancellous Bone Immunohistochemistry Biological Markers ab: Purpose. Blood vessels and skeleton interact together. Endothelin-1 is a potent vasoconstrictor and also has an effect on bone metabolism. The dual antagonist to both endothelin-1 type A and B receptors, Macitentan, has been approved for clinical management of pulmonary arterial hypertension while little is known about the secondary effect of the drug on spine. We aimed to answer how vertebral bone mass responded to Macitentan treatment in mice. Methods. Sixteen male balb/c mice at 6 months were randomly assigned into 2 groups. Vehicle and Macitentan were administrated via intraperitoneal injection to Control group and Treatment group, respectively, for 4 months. At sacrifice, plasma endothelin-1 was evaluated with ELISA and vertebral bone mass was evaluated with Microcomputed Tomography and histological analysis. Results. We found higher plasma endothelin-1 level (p<0.01) and less vertebral bone mass (p<0.05) in Treatment group compared to controls. Moreover, less osteoblasts and more osteoclasts were observed in the vertebral trabecular bone in the Treatment group compared to controls, by immunohistochemistry of the cell-specific markers. Conclusions. Treatment with Macitentan is associated with significant lower vertebral bone mass and therefore the secondary effect of dual antagonists to endothelin-1 receptors on the skeleton should be monitored and investigated in clinical practice. Both osteoblasts and osteoclasts may be involved while the molecular mechanism needs to be further explored. pubtype: Academic Journal doctype: diagnostic images pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|