Clinical relevance of single nucleotide polymorphisms in the CXCL1 and CXCL12 genes in patients with major trauma.
Background: Genetic backgrounds have been recognized as significant determinants of susceptibility to sepsis. CXC chemokines play a significant role in innate immunity against infectious diseases. Genetic polymorphisms of CXC chemokine genes have been widely studied in inflammatory and infectious di...
| Publicado en: | Journal of Trauma & Acute Care Surgery Vol. 86; no. 3; pp. 440 - 448 |
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| Autores principales: | , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Lippincott Williams & Wilkins
Mar2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=134991218&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 134991218 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 21630755 EUMW jtl: Journal of Trauma & Acute Care Surgery issn: 21630755 maglogo: N pubinfo: dt: Mar2019 vid: 86 iid: 3 pid: 433 pub: Lippincott Williams & Wilkins place: Baltimore, Maryland artinfo: ui: 134991218 134991218 NLM30489503 134991218 10.1097/TA.0000000000002141 NLM30489503 134991218 ppf: 440 ppct: 8 formats: tig: atl: Clinical relevance of single nucleotide polymorphisms in the CXCL1 and CXCL12 genes in patients with major trauma. aug: au: Wang, Xiao Zhang, An-Qiang Gu, Wei Wen, Da-Lin Lu, Hong-Xiang Yang, Jian-Hua Wang, Xu Deng, Jin Zhang, Hong-Qi Du, Ding-Yuan Zhang, Lian-Yang Zeng, Ling Jiang, Jian-Xin affil: From the State Key Laboratory of Trauma, Burns and Combined Injury (X.W., A.-Q.Z., W.G., D.-L.W., H.-X.L., J.-H.Y., L.-Y.Z., L.Z., J.-X.J.), Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing sug: subj: Cytokines Sepsis Wounds and Injuries Polymorphism, Genetic Prospective Studies Risk Factors Adult Genotype China Female Human Disease Susceptibility Male Validation Studies Comparative Studies Evaluation Research Multicenter Studies Adult: 19-44 years Female Male ab: Background: Genetic backgrounds have been recognized as significant determinants of susceptibility to sepsis. CXC chemokines play a significant role in innate immunity against infectious diseases. Genetic polymorphisms of CXC chemokine genes have been widely studied in inflammatory and infectious diseases but not in sepsis. Thus, we aimed to investigate the clinical relevance of CXC chemokine gene polymorphisms and susceptibility to sepsis in a traumatically injured population.Methods: Thirteen tag single nucleotide polymorphisms were selected from CXC chemokine genes using a multimarker tagging algorithm in the Tagger software. Three independent cohorts of injured patients (n = 1700) were prospectively recruited. Selected single nucleotide polymorphisms were genotyped using an improved multiplex ligation detection reaction method. Cytokine production in lipopolysaccharide-stimulated whole blood was measured using an enzyme-linked immunosorbent assay.Results: Among the 13 tag single nucleotide polymorphisms, four single nucleotide polymorphisms (rs1429638, rs266087, rs2297630, and rs2839693) were significantly associated with the susceptibility to sepsis, and three (rs3117604, rs1429638, and rs4074) were significantly associated with an increased multiple organ dysfunction score in the derivation cohort. However, only the clinical relevance of rs1429638 and rs266087 was confirmed in the validation cohorts. In addition, rs2297630 was significantly associated with interleukin 6 production.Conclusion: The rs1429638 polymorphism in the CXCL1 gene and the rs2297630 polymorphism in the CXCL12 gene were associated with altered susceptibility to sepsis and might be used as important genetic markers to assess the risks of sepsis in trauma patients.Level Of Evidence: Prognostic and epidemiologic study, level II. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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