Clinical Utility of the Combined Positive Score for Programmed Death Ligand-1 Expression and the Approval of Pembrolizumab for Treatment of Gastric Cancer.

Context.--Regulatory approval of pembrolizumab for treatment of gastric and gastroesophageal junction (G/GEJ) adenocarcinoma required a reproducible scoring method for use of programmed death ligand-1 (PD-L1) protein expression as a companion diagnostic to identify likely responders to therapy. Obje...

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Published in:Archives of Pathology & Laboratory Medicine Vol. 143; no. 3; pp. 330 - 338
Main Authors: Kulangara, Karina, Zhang, Nancy, Corigliano, Ellie, Guerrero, Lindsay, Waldroup, Stephanie, Jaiswal, Dipeshkumar, Jansson, Malinka, Shah, Supriya, Hanks, Debra, Jiangdian Wang, Lunceford, Jared, Savage, Mary J., Juco, Jonathan, Emancipator, Kenneth
Format: equations & formulas pictorial research tables/charts Journal Article
Published: College of American Pathologists Mar2019
Online Access:View this record in EBSCOhost
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      dt: Mar2019
      vid: 143
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      pub: College of American Pathologists
      place: Northfield, Illinois
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        10.5858/arpa.2018-0043-OA
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        atl: Clinical Utility of the Combined Positive Score for Programmed Death Ligand-1 Expression and the Approval of Pembrolizumab for Treatment of Gastric Cancer.
      aug:
        au:
          Kulangara, Karina
          Zhang, Nancy
          Corigliano, Ellie
          Guerrero, Lindsay
          Waldroup, Stephanie
          Jaiswal, Dipeshkumar
          Jansson, Malinka
          Shah, Supriya
          Hanks, Debra
          Jiangdian Wang
          Lunceford, Jared
          Savage, Mary J.
          Juco, Jonathan
          Emancipator, Kenneth
        affil: Division of Companion Diagnostics, Dako North America, Agilent Technologies, Carpinteria, California
      sug:
        subj:
          Stomach Neoplasms Drug Therapy
          Antibodies, Monoclonal Therapeutic Use
          Immunohistochemistry
          Antigens, Surface
          Gene Expression
          Human
          Antibodies, Monoclonal Immunology
          Algorithms
          Biological Markers
          Drug Approval
          Odds Ratio
          Confidence Intervals
          Prospective Studies
      ab: Context.--Regulatory approval of pembrolizumab for treatment of gastric and gastroesophageal junction (G/GEJ) adenocarcinoma required a reproducible scoring method for use of programmed death ligand-1 (PD-L1) protein expression as a companion diagnostic to identify likely responders to therapy. Objective.--To develop an immunohistochemical scoring algorithm that includes PD-L1 expression for tumor and immune cells, that is, the combined positive score. Design.--Four previously treated tumor types in the KEYNOTE-012 and KEYNOTE-028 studies were analyzed descriptively with a version of the PD-L1 immunohistochemical 22C3 pharmDx assay labeled for investigational use only to determine the relative importance of PD-L1 expression in tumor versus immune cells as a biomarker for pembrolizumab response. A combined positive score was developed as a novel scoring method and was compared with the tumor proportion score in cohort 1 from the KEYNOTE-059 study (G/GEJ cancer). External reproducibility was assessed. Results.--Per combined positive score cutoff of 1 or more, the prevalence of PD-L1 expression in patients with G/GEJ cancer was 57.6% (148 of 257 patients), with reasonable enrichment of responses (odds ratio, 2.8). Per tumor proportion score cutoff of 1% or more, prevalence was 12.5% (32 of 257 patients), with minimal enrichment (odds ratio, 1.4). External reproducibility assessments demonstrated interpathologist overall agreement of 96.6% (591 of 612; 95% CI, 94.0%--98.7%) and intrapathologist overall agreement of 97.2% (595 of 612; 95% CI, 95.3%--98.9%). Conclusions.--Combined positive score is a robust, reproducible PD-L1 scoring method that predicts response to pembrolizumab in patients with G/GEJ cancer. This novel scoring method supported US Food and Drug Administration approval of pembrolizumab as third-line therapy for G/GEJ cancer and has facilitated investigation in other indications.
      pubtype: Academic Journal
      doctype:
        equations & formulas
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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