Automatic cell segmentation in histopathological images via two-staged superpixel-based algorithms.

The analysis of cell characteristics from high-resolution digital histopathological images is the standard clinical practice for the diagnosis and prognosis of cancer. Yet, it is a rather exhausting process for pathologists to examine the cellular structures manually in this way. Automating this ted...

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Publicado en:Medical & Biological Engineering & Computing Vol. 57; no. 3; pp. 653 - 666
Autores principales: Albayrak, Abdulkadir, Bilgin, Gokhan
Formato: Journal Article
Publicado: Springer Nature Mar2019
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Mar2019
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      pub: Springer Nature
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        atl: Automatic cell segmentation in histopathological images via two-staged superpixel-based algorithms.
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        au:
          Albayrak, Abdulkadir
          Bilgin, Gokhan
        affil: Department of Computer Engineering, Yildiz Technical University (YTU), 34220, Istanbul, Turkey
      sug:
        subj:
          Kidney Neoplasms Pathology
          Histocytological Preparation Techniques Methods
          Algorithms
          Image Processing, Computer Assisted Methods
          Carcinoma, Renal Cell Pathology
          Cluster Analysis
          Resource Databases
          Scales
      ab: The analysis of cell characteristics from high-resolution digital histopathological images is the standard clinical practice for the diagnosis and prognosis of cancer. Yet, it is a rather exhausting process for pathologists to examine the cellular structures manually in this way. Automating this tedious and time-consuming process is an emerging topic of the histopathological image-processing studies in the literature. This paper presents a two-stage segmentation method to obtain cellular structures in high-dimensional histopathological images of renal cell carcinoma. First, the image is segmented to superpixels with simple linear iterative clustering (SLIC) method. Then, the obtained superpixels are clustered by the state-of-the-art clustering-based segmentation algorithms to find similar superpixels that compose the cell nuclei. Furthermore, the comparison of the global clustering-based segmentation methods and local region-based superpixel segmentation algorithms are also compared. The results show that the use of the superpixel segmentation algorithm as a pre-segmentation method improves the performance of the cell segmentation as compared to the simple single clustering-based segmentation algorithm. The true positive ratio (TPR), true negative ratio (TNR), F-measure, precision, and overlap ratio (OR) measures are utilized as segmentation performance evaluation. The computation times of the algorithms are also evaluated and presented in the study. Graphical Abstract The visual flowchart of the proposed automatic cell segmentation in histopathological images via two-staged superpixel-based algorithms.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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