Micro-CT myelography using contrast-enhanced digital subtraction: feasibility and initial results in healthy rats.
Purpose: The spinal subarachnoid space (SSAS) is vital for neural performance. Although models of spinal diseases and trauma are used frequently, no methods exist to obtain high-resolution myelograms in rodents. Thereby, our aim was to explore the feasibility of obtaining high-resolution micro-CT my...
| Publicado en: | Neuroradiology Vol. 61; no. 3; pp. 323 - 331 |
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| Autores principales: | , , , , , , , |
| Formato: | diagnostic images equations & formulas research tables/charts Journal Article |
| Publicado: |
Springer Nature
Mar2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=135115194&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 135115194 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00283940 NYZ jtl: Neuroradiology issn: 00283940 maglogo: N pubinfo: dt: Mar2019 vid: 61 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 135115194 135115194 135115194 10.1007/s00234-019-02162-8 135115194 ppf: 323 ppct: 8 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Micro-CT myelography using contrast-enhanced digital subtraction: feasibility and initial results in healthy rats. aug: au: Zambrano-Rodríguez, Pablo C. Bolaños-Puchet, Sirio Reyes-Alva, Horacio J. García-Orozco, Luis E. Romero-Piña, Mario E. Martinez-Cruz, Angelina Guízar-Sahagún, Gabriel Medina, Luis A. affil: Department of Neurology, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, 50090, Toluca, Mexico sug: subj: Myelography Methods Tomography, X-Ray Computed Methods Angiography, Digital Subtraction Methods Meninges Radiography Animal Studies Rats Protocols Iodine Nanoparticles Gold Qualitative Studies Quantitative Studies ab: Purpose: The spinal subarachnoid space (SSAS) is vital for neural performance. Although models of spinal diseases and trauma are used frequently, no methods exist to obtain high-resolution myelograms in rodents. Thereby, our aim was to explore the feasibility of obtaining high-resolution micro-CT myelograms of rats by contrast-enhanced dual-energy (DE) and single-energy (SE) digital subtraction.Methods: Micro-CT contrast-enhanced DE and SE imaging protocols were implemented with live adult rats (total of 18 animals). For each protocol, contrast agents based on iodine (Iomeron® 400 and Fenestra® VC) and gold nanoparticles (AuroVist™ 15 nm) were tested. For DE, images at low- and high-energy settings were acquired after contrast injection; for SE, one image was acquired before and the other after contrast injection. Post-processing consisted of region of interest selection, image registration, weighted subtraction, and longitudinal alignment.Results: High-resolution myelograms were obtained with contrast-enhanced digital subtraction protocols. After qualitative and quantitative (contrast-to-noise ratio) analyses, we found that the SE acquisition protocol with Iomeron® 400 provides the best images. 3D contour renderings allowed visualization of SSAS and identification of some anatomical structures within it.Conclusion: This in vivo study shows the potential of SE contrast-enhanced myelography for imaging SSAS in rat. This approach yields high-resolution 3D images without interference from adjacent anatomical structures, providing an innovative tool for further assessment of studies involving rat SSAS. pubtype: Academic Journal doctype: diagnostic images equations & formulas research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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