Assessment of Plant Growth Promoting and Abiotic Stress Tolerance Properties of Wheat Endophytic Fungi.

The aims of the present work were to isolate and characterize fungal endophytic communities associated with healthy wheat (Triticum aestivum L.) plants, collected from the North China. Segregated endophytes were screened for their PGP traits, abiotic stresses (heavy metals, salinity, drought, and te...

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Publicado en:BioMed Research International pp. 1 - 13
Autores principales: Ripa, Farhana Alam, Cao, Wei-dong, Tong, Shuai, Sun, Jian-guang
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 3/27/2019
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 3/27/2019
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1155/2019/6105865
        135573295
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        atl: Assessment of Plant Growth Promoting and Abiotic Stress Tolerance Properties of Wheat Endophytic Fungi.
      aug:
        au:
          Ripa, Farhana Alam
          Cao, Wei-dong
          Tong, Shuai
          Sun, Jian-guang
        affil: Key Laboratory of Microbial Resources, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China
      sug:
        subj:
          Plant Physiology
          Stress, Physiological
          Fungi Physiology
          Fungi Analysis
          Wheat
          Human
          Metals, Heavy
          Osmolar Concentration
          Temperature
          Microbial Culture and Sensitivity Tests
          Sequence Analysis Methods
          Carboxylic Acids
          Indoles
          Acetic Acid
          Phosphates
          Chelating Agents
          Ammonia
          Salt Stress
          Catalase
          Hydrogen Cyanide
          Drought Resistance
      ab: The aims of the present work were to isolate and characterize fungal endophytic communities associated with healthy wheat (Triticum aestivum L.) plants, collected from the North China. Segregated endophytes were screened for their PGP traits, abiotic stresses (heavy metals, salinity, drought, and temperature), and antibiotic sensitivity. A total of 16 endophytic fungi were isolated using the culture-dependent approach from different tissue parts of wheat plants. Based upon their internal transcribed spacer (ITS) rDNA gene sequencing, 15 out of 16 isolates were selected for further analysis. In the contemporary investigation, a number of the tested endophytes exhibited fairly good 1-aminocyclopropane-1-carboxylic acid deaminase (ACCD) (0.03±0.011 to 1.43±0.01 µmol α-KB mg−1 protein hr−1), indole acetic acid (IAA) (1.125±0.04 to36.12±0.004µgml−1), and phosphate solubilizing index (PSI) (2.08±0.03to5.16±0.36) activities. More than 30% isolates gave positive result for siderophore and ammonia tests, whereas all exhibited catalase activity but only 2 (582PDA1 and 582PDA11) produced hydrogen cyanide. Trichoderma strains showed salt, heavy metals, and drought tolerance at high levels and also exhibited resistance to all the tested antibiotics. Strain 582PDA4 was found to be the most temperature (55°C) tolerant isolate. The findings of this study indicated that the microbial endophytes isolated from wheat plants possessing a crucial function to improve plant growth could be utilized as biofertilizers or bioagents to establish a sustainable crop production system.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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